Bell M V, Dick J R, Buda C
Department of Biological and Molecular Sciences, University of Stirling, Scotland.
Lipids. 1997 Oct;32(10):1085-91. doi: 10.1007/s11745-997-0140-y.
The molecular species compositions of the main diacyl phosphoglyceride classes and ether-linked subclasses from sperm of three species of fish, sea bass Dicentrarchus labrax, Atlantic salmon Salmo salar and Chinook salmon Onchorhynchus tsawytscha, were determined. The phospholipids from sperm were highly unsaturated, dipolyunsaturated fatty acid (diPUFA) molecular species comprised 64.6 to 71.8% of phosphatidylserine (PS), 10.1 to 17.4% of phosphatidylethanolamine (PE), and 3.3 to 10.1% of phosphatidylcholine (PC). In sea bass sperm, di22:6n-3 phospholipid was the predominant diPUFA molecular species, but in both salmon species 22:5n-3/22:6n-3 was also a major constituent of PS. Phospholipids containing 22:6n-3 dominated in sea bass sperm with 16:0/22:6n-3 as a major component of PC and PE, and 18:0/22:6n-3 of PE and PS in addition to di22:6n-3 in the latter two classes. In contrast, both salmon species contained much more 20:5n-3 and less 22:6n-3 so that saturated/20:5n-3 and monounsaturated/20:5n-3 molecular species were more abundant than the corresponding molecules containing 22:6n-3. Ether-linked lipids comprised 11.3-36.3% of choline and ethanolamine phosphoglycerides in each fish species. Molecular species containing 22:6n-3 were the major components of 1-O-alkyl-2-acyl-glycerophosphocholine, especially 16:0a/22:6n-3 in sea bass and 18:1a/22:6n-3 in the two salmon species, while in 1-O-alk-1'-enyl-2-acyl-glycerophosphoethanolamine, 16:0a/22:6n-3 was the major component in both salmon and 18:0a/22:6n-3 in sea bass with 18:1a/22:6n-3 abundant in all three species. In Atlantic salmon 1-O-alkyl-2-acyl-glycerophosphoethanolamine comprised 24.6% of ethanolamine glycerophospholipids which were predominantly 16:0a/22:6n-3 and 18:1a/22:6n-3. Phosphatidylinositol from sperm was dominated by stearoyl/C20 PUFA molecular species, in sea bass overwhelmingly 18:0/20:4n-6, while in both salmon species 18:0/20:4n-6 and 18:0/20:5n-3 were equally abundant.
测定了三种鱼类（海鲈、大西洋鲑和奇努克鲑）精子中主要二酰基磷酸甘油酯类和醚键连接亚类的分子种类组成。精子中的磷脂高度不饱和，二聚不饱和脂肪酸（diPUFA）分子种类占磷脂酰丝氨酸（PS）的64.6%至71.8%、磷脂酰乙醇胺（PE）的10.1%至17.4%以及磷脂酰胆碱（PC）的3.3%至10.1%。在海鲈精子中，二二十二碳六烯酸（di22:6n-3）磷脂是主要的diPUFA分子种类，但在两种鲑鱼中，22:5n-3/22:6n-3也是PS的主要成分。含有22:6n-3的磷脂在海鲈精子中占主导地位，16:0/22:6n-3是PC和PE的主要成分，除了后两类中的di22:6n-3外，18:0/22:6n-3是PE和PS的主要成分。相比之下，两种鲑鱼含有更多的20:5n-3和更少的22:6n-3，因此饱和/20:5n-3和单不饱和/20:5n-3分子种类比相应的含22:6n-3分子更丰富。醚键连接的脂质占每种鱼类胆碱和乙醇胺磷酸甘油酯的11.3 - 36.3%。含有22:6n-3的分子种类是1-O-烷基-2-酰基-甘油磷酸胆碱的主要成分，特别是海鲈中的16:0a/22:6n-3和两种鲑鱼中的18:1a/22:6n-3，而在1-O-烯基-2-酰基-甘油磷酸乙醇胺中，16:0a/22:6n-3是两种鲑鱼的主要成分，海鲈中是18:0a/22:6n-3，18:1a/22:6n-3在所有三种鱼类中都很丰富。在大西洋鲑中，1-O-烷基-2-酰基-甘油磷酸乙醇胺占乙醇胺甘油磷脂的24.6%，主要是16:0a/22:6n-3和18:1a/22:6n-3。精子中的磷脂酰肌醇以硬脂酰/C20多不饱和脂肪酸分子种类为主，在海鲈中绝大多数是18:0/20:4n-6，而在两种鲑鱼中则是18:0/20:4n-6和18:0/20:5n-3同样丰富。
