Jansen M W, Brandenburg H, Wildschut H I, Martens A C, Hagenaars A M, Wladimiroff J W, in 't Veld P A
Department of Obstetrics and Gynaecology, Erasmus University, Rotterdam, The Netherlands.
Prenat Diagn. 1997 Oct;17(10):953-9.
Fetal cells are present in the circulation of pregnant women and can be isolated using density gradient centrifugation and magnetic cell sorting. In the present study, maternal cell preparations were depleted for CD45- and CD14-positive cells and enriched for CD71-positive cells. The number of fetal nucleated cells was determined using fluorescence in situ hybridization for X and Y chromosomes. Analysis of maternal blood samples taken before and after transabdominal chorionic villus sampling (TA-CVS) showed an increase in the number of fetal cells in 10 out of 19 male pregnancies after the invasive procedure. This cellular transfusion was found to correlate with elevated maternal serum alpha-fetoprotein levels. TA-CVS-induced cellular transfusion may form a good in vivo system to optimize fetal cell isolation procedures and to study fetal cell dynamics and characteristics.
胎儿细胞存在于孕妇的血液循环中，可通过密度梯度离心和磁性细胞分选进行分离。在本研究中，母体细胞制剂去除了CD45和CD14阳性细胞，并富集了CD71阳性细胞。使用X和Y染色体的荧光原位杂交法测定胎儿有核细胞的数量。对经腹绒毛取样（TA-CVS）前后采集的母体血样分析显示，在19例男性妊娠中，有10例在侵入性操作后胎儿细胞数量增加。发现这种细胞输注与母体血清甲胎蛋白水平升高相关。TA-CVS诱导的细胞输注可能形成一个良好的体内系统，以优化胎儿细胞分离程序，并研究胎儿细胞动力学和特征。
