免疫亲和色谱法在马尿中地塞米松检测中的应用
Immunoaffinity chromatography in the detection of dexamethasone in equine urine.
作者信息
Ribeiro Neto L M, Salvadori M C, Spinosa H S
机构信息
Departamento de Controle e Pesquisas Antidopagem, Jockey Club de São Paulo, SP, Brazil.
出版信息
J Chromatogr Sci. 1997 Nov;35(11):549-51. doi: 10.1093/chromsci/35.11.549.
Due to the widespread use of dexamethasone in racing horses, mostly in low doses by intra-articular administration for the treatment of inflammatory processes, a method is developed to detect this drug in horse urine samples using liquid-liquid extraction followed by immunoaffinity chromatography. Liquid chromatography with diode-array detection is used for the identification of the drug. The use of immunoaffinity columns enhances the selectivity of the analysis, and the results show that dexamethasone can be detected up to 28 h after intra-articular administration.
由于地塞米松在赛马中广泛使用，主要是通过关节内注射低剂量药物来治疗炎症过程，因此开发了一种方法，通过液液萃取，随后进行免疫亲和色谱法，来检测马尿样中的这种药物。采用带有二极管阵列检测的液相色谱法对该药物进行鉴定。免疫亲和柱的使用提高了分析的选择性，结果表明，关节内给药后28小时仍能检测到地塞米松。
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