Aguilera R, Becchi M, Mateus L, Popot M A, Bonnaire Y, Casabianca H, Hatton C K
Service Central d'Analyse, CNRS, Vernaison, France.
J Chromatogr B Biomed Sci Appl. 1997 Nov 21;702(1-2):85-91. doi: 10.1016/s0378-4347(97)00375-7.
A gas chromatography-combustion-isotope ratio mass spectrometry method for confirmation of hydrocortisone abuse in horseracing and equine sports is proposed. Urinary hydrocortisone was converted to a bismethylenedioxy derivative which presents good gas chromatographic properties and brings an extra carbon contribution of only two carbon atoms. Synthetic hydrocortisone has a different 13C abundance from that of natural urinary horse hydrocortisone and the difference is significant, therefore exogenous and endogenous hydrocortisone can be distinguished.
提出了一种气相色谱-燃烧-同位素比率质谱法，用于确认赛马和马术运动中氢化可的松滥用情况。尿中的氢化可的松被转化为双亚甲二氧基衍生物，该衍生物具有良好的气相色谱特性，且仅增加两个碳原子的额外碳贡献。合成氢化可的松的13C丰度与天然马尿氢化可的松不同，且差异显著，因此可以区分外源性和内源性氢化可的松。
