人（HeLa）细胞与烟草杂种（GGLL）原生质体之间的跨界融合。

Interkingdom fusion between human (HeLa) cells and tobacco hybrid (GGLL) protoplasts.

作者信息

Jones C W, Mastrangelo I A, Smith H H, Liu H Z, Meck R A

出版信息

Science. 1976 Jul 30;193(4251):401-3. doi: 10.1126/science.935875.

Abstract

The fusion of human HeLa cells with tobacco protoplasts has been accomplished with the use of polyethylene glycol. The sequence from heterocellular adherence to heterokaryon formation has been followed with light microscopy and confirmed by autoradiographs of heterokaryons containing unlabeled tobacco nuclei and tritium-labeled HeLa nuclei. The HeLa nucleus retained its integrity in the tobacco cytoplasm up to 6 days after fusion.

摘要

利用聚乙二醇已实现了人类海拉细胞与烟草原生质体的融合。通过光学显微镜追踪了从异细胞黏附到异核体形成的过程，并通过对含有未标记烟草细胞核和氚标记海拉细胞核的异核体进行放射自显影加以证实。在融合后的6天内，海拉细胞核在烟草细胞质中保持完整。