Koulis A, Diss T, Isaacson P G, Dogan A
Department of Histopathology, University College London Medical School, United Kingdom.
Am J Pathol. 1997 Nov;151(5):1353-60.
B-cell lymphomas of mucosa-associated lymphoid tissue invariably contain large numbers of reactive tumor-infiltrating T cells. In the stomach, these lymphomas develop secondary to Helicobacter pylori infection, and clinical and in vitro studies have shown that their growth depends on help provided by H. pylori-specific T cells. In this study we characterized tumor-infiltrating T cells in low- and high-grade B-cell lymphomas of mucosa-associated lymphoid tissue using immunohistochemistry. In most cases, CD4+ T cells dominated and almost all T cells were CD45RO+ memory cells. In 11 of 13 cases studied, the proliferating T cells were CD4+ and no proliferation was observed in the CD8+ subset. In low-grade lymphomas, between 7 and 24% of T cells expressed CD40L whereas no CD40L expression was observed in the majority of high-grade tumors. Examination of homing receptor profile showed that both alpha 4 beta 7 integrin+ and L-selectin+ T cells were present. Examination of T cell diversity by a panel of antibodies against different T-cell receptor V beta regions and by analysis of T-cell receptor genes using polymerase chain reaction suggested that the T cells in these tumors were polyclonal. These results show that low-grade B-cell lymphomas of mucosa-associated lymphoid tissue contain a significant population of activated helper T cells that may be important in supporting tumor growth.
黏膜相关淋巴组织的B细胞淋巴瘤总是含有大量反应性肿瘤浸润T细胞。在胃中，这些淋巴瘤继发于幽门螺杆菌感染，临床和体外研究表明，它们的生长依赖于幽门螺杆菌特异性T细胞提供的辅助。在本研究中，我们使用免疫组织化学对黏膜相关淋巴组织的低级别和高级别B细胞淋巴瘤中的肿瘤浸润T细胞进行了特征分析。在大多数情况下，CD4+ T细胞占主导地位，几乎所有T细胞都是CD45RO+记忆细胞。在13例研究病例中的11例中，增殖的T细胞为CD4+，而CD8+亚群未观察到增殖。在低级别淋巴瘤中，7%至24%的T细胞表达CD40L，而在大多数高级别肿瘤中未观察到CD40L表达。对归巢受体谱的检查表明，α4β7整合素+和L-选择素+ T细胞均存在。通过一组针对不同T细胞受体Vβ区域的抗体以及使用聚合酶链反应分析T细胞受体基因来检查T细胞多样性，结果表明这些肿瘤中的T细胞是多克隆的。这些结果表明，黏膜相关淋巴组织的低级别B细胞淋巴瘤含有大量活化的辅助性T细胞，这些细胞可能在支持肿瘤生长方面具有重要作用。
