Gilbert S C, Plebanski M, Harris S J, Allsopp C E, Thomas R, Layton G T, Hill A V
Wellcome Trust Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, UK.
Nat Biotechnol. 1997 Nov;15(12):1280-4. doi: 10.1038/nbt1197-1280.
Ty virus-like particles consist of a single protein species that can be produced in yeast. Recombinant Ty-VLPs carrying a string of up to 15 defined cytotoxic T lymphocyte (CTL) epitopes from Plasmodium species prime protective CTL responses in mice following a single administration without adjuvant. Effective processing of epitopes from the string was demonstrated in vitro and in vivo and was not affected by flanking sequences.
Ty病毒样颗粒由一种可在酵母中产生的单一蛋白质组成。携带来自疟原虫属多达15个确定的细胞毒性T淋巴细胞（CTL）表位序列的重组Ty病毒样颗粒，在无佐剂单次给药后可在小鼠中引发保护性CTL反应。体外和体内实验均证实了序列中表位的有效加工，且不受侧翼序列的影响。
