核糖体失活蛋白细胞毒性的另一种机制：染色体外DNA的降解。
An additional mechanism of ribosome-inactivating protein cytotoxicity: degradation of extrachromosomal DNA.
作者信息
Nicolas E, Goodyer I D, Taraschi T F
机构信息
Department of Pathology, Anatomy and Cell Biology, Jefferson Medical College, 1020 Locust St., Philadelphia, PA 19107, USA.
出版信息
Biochem J. 1997 Oct 15;327 ( Pt 2)(Pt 2):413-7. doi: 10.1042/bj3270413.
Abstract
Inhibition of protein synthesis by cleavage of the N-glycosidic bond of a specific adenine of 28 S rRNA has been accepted as the mechanism by which plant ribosome-inactivating proteins (RIPs) cause cytotoxicity. The cytotoxic action of gelonin on Plasmodium falciparum malaria parasites appears to occur by a different mechanism. Parasite intoxication, which is manifested by mitochondrial dysfunction and lack of nucleic acid synthesis in the erythrocytic cycle following exposure to the toxin, is caused by the elimination of the parasite 6 kb extrachromosomal (mitochondrial) DNA. This is the first report which demonstrates that the DNA-damaging activities of RIPs observed in vitro can contribute to their cytotoxicity.
摘要
通过切割28 S rRNA特定腺嘌呤的N-糖苷键来抑制蛋白质合成，已被公认为是植物核糖体失活蛋白（RIPs）产生细胞毒性的机制。相思豆毒蛋白对恶性疟原虫的细胞毒性作用似乎是通过不同的机制发生的。寄生虫中毒表现为暴露于毒素后红细胞周期中的线粒体功能障碍和核酸合成缺乏，这是由寄生虫6 kb的染色体外（线粒体）DNA消除所致。这是第一份证明在体外观察到的RIPs的DNA损伤活性可导致其细胞毒性的报告。
相似文献
1
An additional mechanism of ribosome-inactivating protein cytotoxicity: degradation of extrachromosomal DNA.核糖体失活蛋白细胞毒性的另一种机制：染色体外DNA的降解。
Biochem J. 1997 Oct 15;327 ( Pt 2)(Pt 2):413-7. doi: 10.1042/bj3270413.
2
The site of action of six different ribosome-inactivating proteins from plants on eukaryotic ribosomes: the RNA N-glycosidase activity of the proteins.六种来自植物的不同核糖体失活蛋白作用于真核生物核糖体的作用位点：这些蛋白的RNA N-糖苷酶活性。
Biochem Biophys Res Commun. 1988 Feb 15;150(3):1032-6. doi: 10.1016/0006-291x(88)90733-4.
3
Ribosome inactivating protein saporin induces apoptosis through mitochondrial cascade, independent of translation inhibition.核糖体失活蛋白皂草素通过线粒体级联反应诱导细胞凋亡，与翻译抑制无关。
Int J Biochem Cell Biol. 2008;40(12):2880-8. doi: 10.1016/j.biocel.2008.06.004. Epub 2008 Jun 20.
4
Metabolic changes of the malaria parasite during the transition from the human to the mosquito host.疟原虫在从人类宿主向蚊子宿主转变过程中的代谢变化。
Annu Rev Microbiol. 1998;52:561-90. doi: 10.1146/annurev.micro.52.1.561.
5
Cell surface receptor directed targeting of toxin to human malaria parasite, Plasmodium falciparum.细胞表面受体介导毒素靶向人类疟原虫——恶性疟原虫。
FEBS Lett. 1996 Oct 28;396(1):57-61. doi: 10.1016/0014-5793(96)01065-4.
6
Ribosome-inactivating proteins.核糖体失活蛋白
Toxicon. 2004 Sep 15;44(4):371-83. doi: 10.1016/j.toxicon.2004.05.004.
7
High-pressure-liquid-chromatographic and fluorimetric methods for the determination of adenine released from ribosomes by ricin and gelonin.用于测定蓖麻毒素和相思子毒素从核糖体释放的腺嘌呤的高压液相色谱法和荧光法。
Biochem J. 1989 May 1;259(3):639-43. doi: 10.1042/bj2590639.
8
Mitochondrial DNA D-loop as a new target of Saporin 6 nuclease activity.线粒体DNA D环作为皂草素6核酸酶活性的新靶点。
Toxicon. 2005 Mar 15;45(4):475-80. doi: 10.1016/j.toxicon.2004.12.007.
9
Plasmodium falciparum: alterations in organelle transcript abundance during the erythrocytic cycle.恶性疟原虫：红细胞内期循环中细胞器转录本丰度的变化
Exp Parasitol. 1995 May;80(3):430-40. doi: 10.1006/expr.1995.1055.
10
The interaction between "R/S domain" of rat 28S ribosomal RNA and ribosome-inactivating proteins investigated by fusidic acid.用夫西地酸研究大鼠28S核糖体RNA的“R/S结构域”与核糖体失活蛋白之间的相互作用。
Biochem Mol Biol Int. 1995 Nov;37(5):877-83.
引用本文的文献
1
Expression of a Recombinant Anti-HIV and Anti-Tumor Protein, MAP30, in Nicotiana tobacum Hairy Roots: A pH-Stable and Thermophilic Antimicrobial Protein.重组抗HIV和抗肿瘤蛋白MAP30在烟草毛状根中的表达：一种pH稳定且嗜热的抗菌蛋白
PLoS One. 2016 Jul 26;11(7):e0159653. doi: 10.1371/journal.pone.0159653. eCollection 2016.
2
Toxin-based therapeutic approaches.基于毒素的治疗方法。
Toxins (Basel). 2010 Nov;2(11):2519-83. doi: 10.3390/toxins2112519. Epub 2010 Oct 28.
本文引用的文献
1
Direct evidence for the deoxyribonuclease activity of the plant ribosome inactivating protein gelonin.植物核糖体失活蛋白gelonin的脱氧核糖核酸酶活性的直接证据。
FEBS Lett. 1997 Apr 7;406(1-2):162-4. doi: 10.1016/s0014-5793(97)00267-6.
2
Polynucleotide:adenosine glycosidase activity of ribosome-inactivating proteins: effect on DNA, RNA and poly(A).多核苷酸：核糖体失活蛋白的腺苷糖苷酶活性：对DNA、RNA和聚腺苷酸的影响
Nucleic Acids Res. 1997 Feb 1;25(3):518-22. doi: 10.1093/nar/25.3.518.
3
Topological requirements for recognition and cleavage of DNA by ribosome-inactivating proteins.核糖体失活蛋白识别与切割DNA的拓扑学要求。
Biol Chem Hoppe Seyler. 1995 Nov;376(11):637-41. doi: 10.1515/bchm3.1995.376.11.637.
4
Polynucleotide: adenosine glycosidase activity of saporin-L1: effect on DNA, RNA and poly(A).多核苷酸：皂草素-L1的腺苷糖苷酶活性：对DNA、RNA和聚腺苷酸的影响
Biochem J. 1996 Oct 15;319 ( Pt 2)(Pt 2):507-13. doi: 10.1042/bj3190507.
5
DNA-nuclease activity of the single-chain ribosome-inactivating proteins dianthin 30, saporin 6 and gelonin.单链核糖体失活蛋白（石竹素30、肥皂草素6和相思豆毒蛋白）的DNA核酸酶活性
FEBS Lett. 1996 Aug 19;392(1):16-20. doi: 10.1016/0014-5793(96)00776-4.
6
Inhibition of HIV-1 production and selective degradation of viral RNA by an amphibian ribonuclease.一种两栖类核糖核酸酶对HIV-1产生的抑制作用及病毒RNA的选择性降解
J Biol Chem. 1996 Aug 23;271(34):20783-8. doi: 10.1074/jbc.271.34.20783.
7
Role of the N terminus in RNase A homologues: differences in catalytic activity, ribonuclease inhibitor interaction and cytotoxicity.N 端在核糖核酸酶 A 同源物中的作用：催化活性、核糖核酸酶抑制剂相互作用及细胞毒性的差异
J Mol Biol. 1996 Apr 19;257(5):992-1007. doi: 10.1006/jmbi.1996.0218.
8
Recombination associated with replication of malarial mitochondrial DNA.与疟原虫线粒体DNA复制相关的重组
EMBO J. 1996 Feb 1;15(3):684-93.
9
Structural features of Plasmodium cytochrome b that may underlie susceptibility to 8-aminoquinolines and hydroxynaphthoquinones.疟原虫细胞色素b的结构特征可能是对8-氨基喹啉和羟基萘醌易感性的基础。
Mol Biochem Parasitol. 1993 Mar;58(1):33-42. doi: 10.1016/0166-6851(93)90088-f.
10
Immunotoxins: magic bullets or misguided missiles?免疫毒素：神奇子弹还是误导性导弹？
Immunol Today. 1993 Jun;14(6):252-9. doi: 10.1016/0167-5699(93)90041-I.
Zotero 插件