蟋蟀磷酸葡萄糖异构酶基因的表征：系统发育、氨基酸保守性和核苷酸组成分析
Characterization of the phosphoglucose isomerase gene from crickets: an analysis of phylogeny, amino acid conservation and nucleotide composition.
作者信息
Katz L A
机构信息
Section of Ecology and Systematics, Cornell University, Ithaca, New York, USA.
出版信息
Insect Mol Biol. 1997 Nov;6(4):305-18.
Although electrophoretic variation in phosphoglucose isomerase (PGI) is often detected in allozyme studies, the Pgi gene has rarely been characterized. Here, I present the cDNA sequence of the Pgi gene from two species of field cricket, Gryllus pennsylvanicus (U65475) and G. veletis (U65476), in which the PGI protein is suspected of being under balancing selection. Phylogenetic analyses support the conclusion that these sequences are truly cricket Pgi. The cricket amino acid sequences are compared to sequences from other taxa to determine conserved residues that may be essential for the function of the protein. Such analysis is necessary as there is no well-resolved structure of the PGI protein. In addition, the compositional bias of cricket Pgi is different from the other animal Pgi genes characterized to date.
尽管在等位酶研究中经常检测到磷酸葡萄糖异构酶（PGI）的电泳变异，但Pgi基因很少被鉴定。在此，我展示了来自两种田蟋蟀（宾夕法尼亚蟋蟀Gryllus pennsylvanicus，登录号U65475；维氏蟋蟀G. veletis，登录号U65476）的Pgi基因的cDNA序列，其中PGI蛋白被怀疑处于平衡选择之下。系统发育分析支持这些序列是真正的蟋蟀Pgi这一结论。将蟋蟀的氨基酸序列与其他分类群的序列进行比较，以确定可能对蛋白质功能至关重要的保守残基。由于PGI蛋白没有解析良好的结构，这种分析是必要的。此外，蟋蟀Pgi的组成偏差与迄今为止已鉴定的其他动物Pgi基因不同。
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