Faik P, Walker J I, Redmill A A, Morgan M J
Wellcome Research Laboratory for Molecular Genetics, United Medical School, Guy's Hospital, London, UK.
Nature. 1988 Mar 31;332(6163):455-7. doi: 10.1038/332455a0.
Neuroleukin is a neurotrophic factor of relative molecular mass (Mr) 56,000 (56K) found in skeletal muscle, brain, heart and kidneys which supports the survival of embryonic spinal neurones, skeletal motor neurones and sensory neurones. Neuroleukin is also a lymphokine product of lectin-stimulated T cells and induces immunoglobulin secretion by cultured human peripheral blood mononuclear cells. Mouse neuroleukin has been cloned, the complete nucleotide sequence has been determined and its complementary DNA has been transiently expressed in monkey COS-1 cells. The serum-free supernatant of the transfected, but not of control mock-transfected, cells was shown to mimic the properties of neuroleukin isolated from mouse salivary glands. In our work on the molecular genetics of carbohydrate metabolism we have recently isolated a mouse glucose-6-phosphate isomerase (or phosphoglucose isomerase, PGI) cDNA clone using the yeast PGI gene (PGI 1) as a probe. We report here that there is complete sequence identity between the 759 nucleotides at the 3' end of this clone (coding and non-coding) and the sequence of mouse neuroleukin.
神经白细胞素是一种相对分子质量（Mr）为56,000（56K）的神经营养因子，在骨骼肌、脑、心脏和肾脏中发现，它能支持胚胎脊髓神经元、骨骼肌运动神经元和感觉神经元的存活。神经白细胞素也是凝集素刺激的T细胞的淋巴因子产物，并能诱导培养的人外周血单核细胞分泌免疫球蛋白。小鼠神经白细胞素已被克隆，其完整的核苷酸序列已被确定，并且其互补DNA已在猴COS-1细胞中瞬时表达。转染细胞（而非对照假转染细胞）的无血清上清液显示出模拟从小鼠唾液腺分离的神经白细胞素的特性。在我们关于碳水化合物代谢分子遗传学的研究中，我们最近使用酵母磷酸葡萄糖异构酶基因（PGI 1）作为探针分离出了一个小鼠葡萄糖-6-磷酸异构酶（或磷酸葡萄糖异构酶，PGI）cDNA克隆。我们在此报告，该克隆3'端759个核苷酸（编码和非编码）与小鼠神经白细胞素的序列完全相同。
