Hasegawa Y, Negishi S, Naito J, Ishiguro I, Martin G, Juchault P, Katakura Y
Department of Biology, Keio University, Yokohama, Japan.
Pigment Cell Res. 1997 Oct;10(5):265-70. doi: 10.1111/j.1600-0749.1997.tb00686.x.
Genetic studies and quantitative determination of levels of 3-hydroxykynurenine and kynurenine were performed in an albino strain of a terrestrial isopod Armadillidium vulgare. From the results of matings between the albino and the albino, the red, the dark red, or the wild type individuals, the albino A. vulgare seems to be regulated by an autosomal gene(s) recessive to its wild allele. Litter mating of F1 progenies obtained by crossing the albino and the red mutant or the albino and the dark red mutant yielded progenies at a ratio of 3:6:3:4 for the red, the dark red, the wild, and the albino phenotypes, respectively. The albino gene(s) seems not to be allelic but to be epistatic to the red gene(s) with respect to ommochrome biosynthesis. Quantitative determination of 3-hydroxykynurenine carried out by high-performance liquid chromatography with electrochemical detection revealed that the 3-hydroxykynurenine content in the albino was significantly lower than that in the wild or the red type. The whole content of 3-hydroxykynurenine after enzymatic conversion of kynurenine to 3-hydroxykynurenine was still considerably lower than that found in the wild type, even though it increased after the conversion. The albino gene(s) seems to be associated with a blockage at distinct level(s) of ommochrome biosynthesis.
在陆生等足类动物普通鼠妇的一个白化品系中进行了遗传研究以及3-羟基犬尿氨酸和犬尿氨酸水平的定量测定。从白化个体与白化个体、红色个体、深红色个体或野生型个体之间的交配结果来看，白化普通鼠妇似乎受一个对其野生等位基因隐性的常染色体基因调控。白化个体与红色突变体或白化个体与深红色突变体杂交获得的F1后代进行同窝交配，分别产生了红色、深红色、野生型和白化型后代，比例为3:6:3:4。就眼色素生物合成而言，白化基因似乎不是等位基因，而是对红色基因上位。通过高效液相色谱-电化学检测对3-羟基犬尿氨酸进行定量测定，结果显示白化个体中3-羟基犬尿氨酸的含量显著低于野生型或红色型。尽管犬尿氨酸酶促转化为3-羟基犬尿氨酸后其总含量有所增加，但仍远低于野生型中的含量。白化基因似乎与眼色素生物合成的不同水平受阻有关。
