Terenzi F, Falasca L, Marino M, Mele R, Velardi G, Conti Devirgiliis L
Department of Cellular and Developmental Biology, University La Sapienza, Roma, Italy.
Cell Mol Biol (Noisy-le-grand). 1997 Sep;43(6):861-71.
In this work, the proliferative activity of chick embryo hepatocytes and its regulation by factors affecting cell growth, such as epidermal growth factor (EGF) and retinoic acid, were studied. The transport of nutritional molecules, like amino acids, was also investigated; in particular, the uptake mediated by the Na+ -dependent system A, known to be under hormonal and growth factor control. Moreover, some steps in the transduction pathway of mitogenic signal were analyzed, both for EGF and retinoic acid treatment. Results obtained suggest that chick embryo hepatocytes growth in an exclusive serum dependent way shows an early sensibility to EGF stimulation as regards to the proliferative activity and amino acid transport, responds to retinoic acid treatment and lack of contact inhibition. Moreover, as far as the signal transduction is concerned, at early stages, they do not seem to be able to utilize the same signal molecules as observed in adult life.
在本研究中，我们对鸡胚肝细胞的增殖活性及其受影响细胞生长的因子（如表皮生长因子（EGF）和视黄酸）的调控进行了研究。我们还研究了营养分子（如氨基酸）的转运；特别是由已知受激素和生长因子控制的Na⁺依赖性A系统介导的摄取。此外，我们分析了有丝分裂信号转导途径中的一些步骤，包括EGF和视黄酸处理后的情况。所得结果表明，鸡胚肝细胞以完全依赖血清的方式生长，在增殖活性和氨基酸转运方面对EGF刺激表现出早期敏感性，对视黄酸处理有反应且缺乏接触抑制。此外，就信号转导而言，在早期阶段，它们似乎无法利用在成年期观察到的相同信号分子。
