Mazzoli S, Trabattoni D, Lo Caputo S, Piconi S, Blé C, Meacci F, Ruzzante S, Salvi A, Semplici F, Longhi R, Fusi M L, Tofani N, Biasin M, Villa M L, Mazzotta F, Clerici M
Centro M.S.T./U.O. Malattie Infettive, Ospedale S.M. Annunziata, Florence, Italy.
Nat Med. 1997 Nov;3(11):1250-7. doi: 10.1038/nm1197-1250.
HIV-specific mucosal and cellular immunity was analyzed in heterosexual couples discordant for HIV status in serum and in HIV-unexposed controls. HIV-specific IgA but not IgG was present in urine and vaginal wash samples from HIV-exposed seronegative individuals (ESN), whereas both IgA and IgG were observed in their HIV-seropositive partners; antibodies were not detected in low-risk controls. Envelope protein (Env) peptide-stimulated interleukin-2 (IL-2) production by peripheral blood mononuclear cells (PBMCs) was detected in 9 out of 16 ESNs, 5 out of 16 HIV-infected patients and 1 out of 50 controls. Env peptide-stimulated PBMCs of ESNs produced more IL-2 and less IL-10 compared with those of HIV-infected individuals; no differences were observed in chemokine production or in CCR5 expression. These data demonstrate that a compartmentalized immune response to pathogens is possible in humans and raise the possibility of protective roles for cell-mediated immunity and mucosal IgA in HIV-seronegative individuals exposed to HIV.
在血清中HIV状态不一致的异性恋伴侣以及未接触HIV的对照者中，分析了HIV特异性黏膜免疫和细胞免疫。在接触HIV但血清学阴性个体（ESN）的尿液和阴道冲洗样本中存在HIV特异性IgA，但不存在IgG，而在其HIV血清学阳性伴侣中同时观察到了IgA和IgG；在低风险对照者中未检测到抗体。在16名ESN中的9名、16名HIV感染患者中的5名以及50名对照者中的1名中，检测到外周血单个核细胞（PBMC）受包膜蛋白（Env）肽刺激产生白细胞介素-2（IL-2）。与HIV感染个体相比，ESN的Env肽刺激的PBMC产生更多的IL-2和更少的IL-10；在趋化因子产生或CCR5表达方面未观察到差异。这些数据表明，人类对病原体的免疫反应可能存在分区现象，并增加了细胞介导免疫和黏膜IgA在接触HIV的HIV血清学阴性个体中发挥保护作用的可能性。
