Jolly C, Morimoto R, Robert-Nicoud M, Vourc'h C
DyOGen, INSERM U309, Institut Albert Bonniot, Domaine de la Merci, 38706, La Tronche cedex, France.
J Cell Sci. 1997 Dec;110 ( Pt 23):2935-41. doi: 10.1242/jcs.110.23.2935.
In this paper, we show that upon heat shock, HSF1 concentrates in the nucleus of diploid human fibroblasts in two large foci. The relative distribution of HSF1 nuclear foci and active heat shock protein (hsp) genes was investigated by combining fluorescence in situ hybridization (FISH) for the detection of hsp nuclear transcripts and immunofluorescence for the detection of HSF1. We show that the HSF1 foci are distinct from the sites of hsp70 and hsp90 genes transcription. This is the second report of ploidy-dependent foci of transcription factors that are independent of their specific transcription sites. However, the correlation between the number of HSF1 foci and the ploidy of the cells strongly supports the existence of a specific chromosomal target for HSF1 foci.
在本文中，我们表明热休克时，HSF1在二倍体人成纤维细胞核中聚集形成两个大的病灶。通过结合用于检测热休克蛋白（hsp）核转录本的荧光原位杂交（FISH）和用于检测HSF1的免疫荧光，研究了HSF1核病灶与活性热休克蛋白基因的相对分布。我们发现HSF1病灶与hsp70和hsp90基因转录位点不同。这是关于转录因子的倍性依赖性病灶独立于其特定转录位点的第二篇报道。然而，HSF1病灶数量与细胞倍性之间的相关性有力地支持了HSF1病灶存在特定染色体靶点。
