Huang Y, Kawaguchi O, Zeng B, Carrington R A, Horam C J, Yuasa T, Abdul-Hussein N, Hunyor S N
Cooperative Research Centre for Cardiac Technology, Royal North Shore Hospital, St. Leonards (Sydney), Australia.
ASAIO J. 1997 Sep-Oct;43(5):M408-13.
Similarities in coronary circulation and heart size of sheep to that of humans are specific advantages of a sheep model of congestive heart failure (CHF). CHF was created in 11 sheep (51 +/- 4 kg) by selective sequential intracoronary injection of 90 microns microspheres under 1.5% isoflurane anesthesia. Hemodynamic characteristics were assessed at baseline, 4 weeks after establishment of CHF (ejection fraction [EF] < 35%, n = 11), and 26 weeks (n = 7) later. Baseline echocardiographic EF was 59 +/- 5% and fell to 26 +/- 5% after 5 +/- 2 embolizations. The left ventricular (LV) pressure-volume relationship showed stable decreases in LV end-systolic elastance (Ees) and preload recruitable stroke work. Intravenous infusion of dobutamine increased Ees from 2.8 +/- 1.7 to 4.3 +/- 2.2 and 4.5 +/- 1.4 mmHg/ml at heart rates of 140 and 160/min, respectively, at baseline. Increases of Ees (from 1.3 +/- 0.5 to 2.3 +/- 0.7 and 1.9 +/- 0.5 mmHg/ml at heart rates of 140 and 160/min, respectively) with dobutamine under CHF conditions did not exceed Ees values at baseline without dobutamine. This response to dobutamine infusion did not change 26 weeks after establishment of CHF. This stable ovine CHF model is proposed for studies on the long-term effects of cardiac assist devices.
绵羊的冠状动脉循环和心脏大小与人类相似，这是充血性心力衰竭（CHF）绵羊模型的特定优势。在1.5%异氟烷麻醉下，通过选择性顺序冠状动脉内注射90微米微球，在11只绵羊（51±4千克）中建立CHF。在基线、CHF建立后4周（射血分数[EF]<35%，n=11）和26周后（n=7）评估血流动力学特征。基线超声心动图EF为59±5%，在5±2次栓塞后降至26±5%。左心室（LV）压力-容积关系显示LV收缩末期弹性（Ees）和预负荷可募集搏功稳定下降。静脉输注多巴酚丁胺在基线时分别使心率为140和160次/分钟时的Ees从2.8±1.7增加到4.3±2.2和4.5±1.4 mmHg/ml。在CHF条件下，多巴酚丁胺使Ees增加（分别在心率为140和160次/分钟时从1.3±0.5增加到2.3±0.7和1.9±0.5 mmHg/ml），但不超过基线时无多巴酚丁胺时的Ees值。CHF建立26周后，对多巴酚丁胺输注的这种反应没有改变。该稳定的绵羊CHF模型被提议用于研究心脏辅助装置的长期效果。
