Konishi H, Sohara Y, Endo S, Misawa Y, Fuse K
Department of Thoracic and Cardiovascular Surgery, Jichi Medical School, Tochigi, Japan.
ASAIO J. 1997 Sep-Oct;43(5):M657-9.
During development of a rotary blood pump as an assist device, the efficacy of non pulsatile perfusion to the end organ has to be verified. However, there are few evaluations of two different perfusion mechanisms through the tissue microcirculation. In this study, the pulmonary microcirculation was analyzed by vital microscopic observation. Wistar rats weighing 400-500 g were anesthetized and ventilated by a respirator. After establishing right heart bypass from the right atrium to the pulmonary artery using a roller pump, the pulmonary microcirculation was observed during intravenous infusion of bovine albumin tagged with fluorescein isothiocyanate. The images were recorded on a videotape through an ultra-sensitive SIT TV camera. Initially, the pulmonary circulation was pulsatile, produced by the native heart, and the pulmonary capillary network was evenly perfused by the blood. After starting the pump, the flow became non pulsatile and the distribution of capillary perfusion was displaced to the short circuit connecting the pulmonary arterioles and venules. Flow distribution during non pulsatile perfusion was heterogeneous compared with pulsatile perfusion. This result suggests that non pulsatile flow may lead to the deterioration of function. Further investigation is necessary to evaluate the relationship between the microcirculation and organ function.
在研发作为辅助装置的旋转式血泵过程中，必须验证对终末器官进行非搏动性灌注的效果。然而，很少有对通过组织微循环的两种不同灌注机制的评估。在本研究中，通过活体显微镜观察对肺微循环进行了分析。将体重400 - 500克的Wistar大鼠麻醉并用呼吸机通气。使用滚压泵建立从右心房到肺动脉的右心旁路后，在静脉输注异硫氰酸荧光素标记的牛白蛋白期间观察肺微循环。图像通过超灵敏SIT电视摄像机记录在录像带上。最初，肺循环是由天然心脏产生的搏动性循环，肺毛细血管网络被血液均匀灌注。启动血泵后，血流变为非搏动性，毛细血管灌注分布转移到连接肺小动脉和小静脉的短路处。与搏动性灌注相比，非搏动性灌注期间的血流分布不均匀。这一结果表明非搏动性血流可能导致功能恶化。有必要进一步研究以评估微循环与器官功能之间的关系。
