Suzuki Y, Tanihara M, Nishimura Y, Suzuki K, Kakimaru Y, Shimizu Y
Department of Plastic and Reconstructive Surgery, Faculty of Medicine, Kyoto University, Japan.
ASAIO J. 1997 Sep-Oct;43(5):M854-7.
The aim of this study was to develop a new wound dressing with controlled release of antibiotics only in the presence of infection. In the first experiment using an infected dorsal pouch of rats, exudate containing proteinases from pouches contaminated with Staphylococcus aureus or Pseudomonas aeruginosa showed significantly higher hydrolytic activity toward Boc-Val-Pro-Arg-MCA than that from noninfected wounds. The authors then developed a new type of wound dressing (AGX), a drug delivery system in which gentamicin is bound to polyvinylalcohol hydrogel through an enzymatically degradable peptide linker containing a -(D)-Phe-Pro-Arg-sequence. To investigate in vitro effectiveness, AGX was incubated with exudate from S. aureus infected or P. aeruginosa infected wounds. Gentamicin was selectively released from AGX in the presence of the exudate from S. aureus infected or P. aeruginosa infected wounds, but was not released in the presence of exudate from noninfected wounds. Next, AGX or the polyvinylalcohol hydrogel that served as control was incubated with S. aureus in the presence of human plasma. After 24 hours, S. aureus concentration was markedly lower in the case with AGX than in that with polyvinylalcohol hydrogel. These results indicate that proteinases from wounds infected with S. aureus or P. aeruginosa cleaved the linker and gentamicin was released.
本研究的目的是开发一种新型伤口敷料，使其仅在感染存在时可控释放抗生素。在第一个实验中，使用感染的大鼠背部皮袋，来自被金黄色葡萄球菌或铜绿假单胞菌污染的皮袋的含有蛋白酶的渗出液对Boc-Val-Pro-Arg-MCA的水解活性明显高于未感染伤口的渗出液。作者随后开发了一种新型伤口敷料（AGX），这是一种药物递送系统，其中庆大霉素通过含有-(D)-Phe-Pro-Arg序列的可酶解肽接头与聚乙烯醇水凝胶结合。为了研究体外有效性，将AGX与金黄色葡萄球菌感染或铜绿假单胞菌感染伤口的渗出液一起孵育。庆大霉素在金黄色葡萄球菌感染或铜绿假单胞菌感染伤口的渗出液存在下从AGX中选择性释放，但在未感染伤口的渗出液存在下不释放。接下来，将AGX或用作对照的聚乙烯醇水凝胶与人血浆一起与金黄色葡萄球菌孵育。24小时后，使用AGX的情况下金黄色葡萄球菌浓度明显低于使用聚乙烯醇水凝胶的情况。这些结果表明，来自金黄色葡萄球菌或铜绿假单胞菌感染伤口的蛋白酶切割接头并释放庆大霉素。
