Grzybowski J, Antos-Bielska M, Ołdak E, Trafny E A
Department of Microbiology, Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
Polim Med. 1997;27(3-4):3-9.
Our new collagen dressing has been developed recently. Three types (A, B, and C) of the dressing were prepared in this study. Each type contained bacitracin, neomycin or colistin. The antibiotic was input into: i. collagen sponge (CS)--type A, ii. layer of limited hydrophobicity (LLH)--type B, and iii. into both CS and LLH layers--type C. The final concentration of the antibiotic that resulted from the loading level was 2 mg/cm2 for the dressings of type A and B and 4 mg/cm2 for the dressing of type C. The antibiotics were then extracted from the pieces of dressings for two days through dialysis membrane. Susceptibility of 54 bacterial strains (S. aureus, P. aeruginosa, and Acinetobacter) isolated from burn wounds were tested to the three antibiotics used for preparation of the dressings. The results of the study evidenced that efficiency of released of antibiotics into the extracts depended on the kind of antibiotic and on the type of dressing. The concentration of the antibiotics proved to be much higher than MIC90 values of the bacterial isolates tested in respect to their susceptibility. The dressing containing mixture of the three antibiotics in two layers--CS and LLH is now considered as potentially effective for care of infected wounds. It may be useful for the treatment of infected wounds or for profilaxis of contaminated wounds, ensuring: i. sufficient antimicrobial activity in wound, and ii. optimal wound environment for the presence of collagenic biomaterial on the damaged tissue.
我们的新型胶原蛋白敷料是最近研发出来的。本研究制备了三种类型（A、B和C）的敷料。每种类型都含有杆菌肽、新霉素或黏菌素。抗生素被加入到：i. 胶原蛋白海绵（CS）——A型，ii. 有限疏水性层（LLH）——B型，以及iii. 同时加入到CS层和LLH层——C型。根据负载水平，敷料中抗生素的最终浓度对于A型和B型敷料为2 mg/cm²，对于C型敷料为4 mg/cm²。然后通过透析膜从敷料片中提取抗生素两天。对从烧伤创面分离出的54株细菌（金黄色葡萄球菌、铜绿假单胞菌和不动杆菌）对用于制备敷料的三种抗生素的敏感性进行了测试。研究结果表明，抗生素向提取物中的释放效率取决于抗生素的种类和敷料的类型。抗生素的浓度被证明远高于所测试细菌分离株的MIC90值。含有三种抗生素混合物的双层（CS和LLH）敷料目前被认为对感染伤口的护理可能有效。它可能对感染伤口的治疗或污染伤口的预防有用，可确保：i. 在伤口中有足够的抗菌活性，以及ii. 为受损组织上存在的胶原生物材料提供最佳的伤口环境。
