Winn H N, Todd H M, Amon E, al-Malt A, Molnár M, Hertelendy F
Department of Obstetrics and Gynecology, St. Louis University Health Sciences Center, Missouri, USA.
J Matern Fetal Med. 1997 Sep-Oct;6(5):249-53. doi: 10.1002/(SICI)1520-6661(199709/10)6:5<249::AID-MFM1>3.0.CO;2-G.
There is growing evidence that proteinuric hypertension of pregnancy (preeclampsia) is associated with endothelial dysfunction. The aim of this study was to evaluate the effects of serum from preeclamptic patients on basal and agonist-stimulated prostacyclin production by human umbilical vein endothelial cells (HUVEC) in culture and to compare these to the effects of serum from normal pregnant and nonpregnant women. During a 24 h incubation of HUVEC with 20% of preeclampsia serum, baseline prostacyclin output was significantly (P < 0.01) increased over the control groups. However, this response was attenuated by extending the exposure to 72 h. Histamine, thrombin and the calcium ionophore, A23187, all acutely increased prostacyclin production, but the increase relative to baseline levels was greatest in HUVEC preincubated for 24 h in normal serum transiently promotes prostacyclin production in HUVEC derived from normal pregnancies, preeclampsia serum transiently promotes prostacyclin production in HUVEC derived from normal pregnancies, and 2) the relative increase in response to agonists is reduced by preeclampsia serum, compared to normal pregnancy sera.
越来越多的证据表明，妊娠期蛋白尿性高血压（先兆子痫）与内皮功能障碍有关。本研究的目的是评估先兆子痫患者血清对培养的人脐静脉内皮细胞（HUVEC）基础和激动剂刺激的前列环素产生的影响，并将其与正常孕妇和非孕妇血清的影响进行比较。在HUVEC与20%先兆子痫血清孵育24小时期间，基线前列环素产量比对照组显著增加（P<0.01）。然而，将暴露时间延长至72小时会减弱这种反应。组胺、凝血酶和钙离子载体A23187均可急性增加前列环素的产生，但在正常血清中预孵育24小时的HUVEC中，相对于基线水平的增加最大。正常血清可短暂促进源自正常妊娠的HUVEC中前列环素的产生，先兆子痫血清可短暂促进源自正常妊娠的HUVEC中前列环素的产生，并且2）与正常妊娠血清相比，先兆子痫血清可降低对激动剂反应的相对增加。
