来自红球菌属LK2的位点特异性内切酶RspLKI和RspLKII是SphI和BamHI的同裂酶。
Site-specific endonucleases RspLKI and RspLKII from Rhodococcus species LK2 are isoschizomers of SphI and BamHI.
作者信息
Zabaznaya E V, Zheleznaya L A, Matvienko N I
机构信息
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
出版信息
Biochemistry (Mosc). 1997 Aug;62(8):872-82.
Two site-specific endonucleases, RspLKI and RspLKII, have been isolated and purified to functional homogeneity from the soil bacterium Rhodococcus species LK2. RspLKI recognizes the 5'-GCATG decreases C-3' DNA sequence and RspLKII recognizes the 5'-G decreases GATCC-3' sequence (arrows indicate DNA cleavage sites). The isolated enzymes are class II site specific endonucleases and are isoschizomers of endonucleases SphI and BamHI, respectively.
已从土壤细菌红球菌属LK2中分离并纯化出两种位点特异性内切酶RspLKI和RspLKII，使其达到功能均一性。RspLKI识别5'-GCATG↓C-3' DNA序列，RspLKII识别5'-G↓GATCC-3'序列（箭头指示DNA切割位点）。分离出的酶属于II类位点特异性内切酶，分别是内切酶SphI和BamHI的同裂酶。
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