Puchkova L V, Zakharova E T, Aleinikova T D, Mokshina S V, Tsymbalenko N V, Sasina L K, Shirmanova M R, Rogacheva N P, Gaitskhoki V S
Research Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg, Russia.
Biochemistry (Mosc). 1997 Aug;62(8):928-30.
The content of ceruloplasmin (Cp) was determined in 96 samples of human breast milk using rocket immunoelectrophoresis and measurement of Cp oxidase activity. The concentration of immunoreactive Cp in milk decreased about 9 times during the first 20 days of lactation while the specific oxidase activity decreased only 4 times. Two-dimensional electrophoresis of purified milk Cp before and after its treatment with chelating agents showed that copper atoms in milk Cp are more sensitive to EDTA treatment that those in blood Cp. The comparison of the different lectin-binding ability of blood and milk Cp's revealed a difference in the composition of their carbohydrate chains. The mechanisms controlling the uptake of copper ions by newborns at the level of the expression of the Cp-encoding gene in the mammary gland of the mother are discussed.
采用火箭免疫电泳法和铜蓝蛋白氧化酶活性测定法，对96份人母乳样本中的铜蓝蛋白（Cp）含量进行了测定。哺乳期前20天，母乳中免疫反应性Cp的浓度下降了约9倍，而比氧化酶活性仅下降了4倍。用螯合剂处理前后，对纯化的母乳Cp进行二维电泳分析，结果表明，母乳Cp中的铜原子比血液Cp中的铜原子对EDTA处理更为敏感。对血液和母乳Cp的不同凝集素结合能力进行比较，结果显示二者碳水化合物链的组成存在差异。本文还讨论了在母亲乳腺中，Cp编码基因表达水平上控制新生儿铜离子摄取的机制。
