Braun P, Guttman F M, Berdnikoff G, Sandborn E B, Morin C
Surg Gynecol Obstet. 1976 Jul;143(1):43-50.
Morphologic changes, resulting from intracellular or extracellular perfusates with dimethyl sulfoxide as a cryophylactic agent, are similar and probably due to the anoxic period, after short term perfusion. These changes are temporary and are followed by complete recovery. No statistically significant changes in disaccharidase activity between intracellular and extracellular perfusates are found in the jejunal mucosa. No differences are found between normal disaccharidase activity and values obtained experimentally. Results of this study support the suggestion that vascular endothelial damage, as observed in evaluations utilizing the electron microscope, is certainly an important factor in the limitation of perfusion techniques of the intestine.
以二甲基亚砜作为冷冻保护剂，经细胞内或细胞外灌注所导致的形态学变化是相似的，可能是由于短期灌注后的缺氧期所致。这些变化是暂时的，随后会完全恢复。在空肠黏膜中，未发现细胞内和细胞外灌注液之间的双糖酶活性有统计学上的显著变化。正常双糖酶活性与实验所得值之间未发现差异。本研究结果支持以下观点：在利用电子显微镜进行的评估中所观察到的血管内皮损伤，肯定是肠道灌注技术受限的一个重要因素。
