Kumano K, Ma P, Hyodo T, Sakai T
Kidney Center, Kitasato University Hospital, Kanagawa, Japan.
Adv Perit Dial. 1997;13:58-63.
Hyaluronan (HA) plays an important role in peritoneal tissue remodeling and the inflammatory process. In this study, attention was directed to the effects of various osmotic agents on HA synthesis in human peritoneal mesothelial cells (HMCs) and fibroblasts (HFBs). Following incubation with growth arrest media for 48 hours, the cells (4 x 10(4)/mL) were incubated for 72 hours in media at various concentrations (50, 100 mmol/L for crystalloid agents and 3.5%, 7% for oncotic agents) of glucose, mannitol, glycerol, sucrose, raffinose, NaCl, combined amino acids, maltodextrin, dextran 70, and hydroxyethylstarch 20. HA synthesis for 72 hours was measured by sandwich-binding protein assay. HMCs synthesized approximately 0.15 ng/cell/72 hours and HFBs 0.09 ng. All crystalloid osmotic agents significantly suppressed HA synthesis by HMCs and HFBs in a concentration-dependent manner. There was no such suppression by any oncotic osmotic agent, which, to the contrary, enhanced the synthesis by 6%-63% in HMCs. In conclusion, HMCs and HFBs may be considered sources of HA in the peritoneal dialysis effluent. Crystalloid osmotic agents suppressed HA synthesis in these cells. Oncotic osmotic agents seem to be less toxic in this regard.
透明质酸（HA）在腹膜组织重塑和炎症过程中发挥着重要作用。在本研究中，我们关注了各种渗透剂对人腹膜间皮细胞（HMCs）和成纤维细胞（HFBs）中HA合成的影响。在用生长停滞培养基孵育48小时后，将细胞（4×10⁴/mL）在含有不同浓度（晶体剂为50、100 mmol/L，胶体剂为3.5%、7%）的葡萄糖、甘露醇、甘油、蔗糖、棉子糖、NaCl、复合氨基酸、麦芽糊精、右旋糖酐70和羟乙基淀粉20的培养基中孵育72小时。通过夹心结合蛋白测定法测量72小时内的HA合成。HMCs合成约0.15 ng/细胞/72小时，HFBs合成0.09 ng。所有晶体渗透剂均以浓度依赖性方式显著抑制HMCs和HFBs的HA合成。任何胶体渗透剂均无此类抑制作用，相反，其可使HMCs中的合成增加6% - 63%。总之，HMCs和HFBs可能被视为腹膜透析流出液中HA的来源。晶体渗透剂抑制这些细胞中的HA合成。在这方面，胶体渗透剂似乎毒性较小。
