Ferens-Sieczkowska M, Orczyk-Pawiłowicz M, Morawiecka B
Institute of Biochemistry and Molecular Biology, University of Wrocław, Poland.
Acta Biochim Pol. 1997;44(2):301-7.
The lectin isolated from the leaves of Iris hybrida binds specifically N-acetyl-galactosamine and lactose. Its molecule consists of two identical subunits bound by disulfide bonds. The lectin is a glycoprotein containing about 12% of sugars. It binds asialoglycoproteins containing complex type sugar chains. The binding is reduced by half at the concentration of 0.15 to 0.40 mM of the galactose containing disaccharides irrespectively to a type of galactose isomer. This indicates rather broad specificity of I. hybrida leaf lectin.
从杂交鸢尾叶片中分离出的凝集素能特异性结合N-乙酰半乳糖胺和乳糖。其分子由通过二硫键结合的两个相同亚基组成。该凝集素是一种糖蛋白，含糖量约为12%。它能结合含有复合型糖链的去唾液酸糖蛋白。无论半乳糖异构体的类型如何，在含有半乳糖的二糖浓度为0.15至0.40 mM时，结合能力会降低一半。这表明杂交鸢尾叶片凝集素具有相当广泛的特异性。
