Lecanda F, Avioli L V, Cheng S L
Department of Internal Medicine, Washington University School of Medicine at Barnes-Jewish Hospital, St. Louis, Missouri 63110, USA.
J Cell Biochem. 1997 Dec 1;67(3):386-96.
We have examined the effects of BMP-2 on the expression of bone matrix proteins in both human bone marrow stromal cells (HBMSC) and human osteoblasts (HOB) and their proliferation and mineralization. Both HBMSC and HOB express BMP-2/-4 type I and type II receptors. Treatment of these two cell types with BMP-2 for 4 weeks in the presence of beta-glycerophosphate and ascorbic acid results in mineralization of their matrix. BMP-2 increases the mRNA level and activities of alkaline phosphatase and elevates the mRNA levels and protein synthesis of osteopontin, bone sialoprotein, osteocalcin, and alpha 1(I) collagen in both cell types. Whereas the mRNA level of decorin is increased, the mRNA concentration of biglycan is not altered by BMP-2. No effect on osteonectin is observed. The effect of BMP-2 on bone matrix protein expression is dose dependent from 25 to 100 ng/ml and is evident after 1-7 days treatment. In the presence of BMP-2, proliferation of HBMSC and HOB is decreased under either serum-free condition or in the presence of serum. Thus, BMP-2 has profound effects on the proliferation, expression of most of the bone matrix proteins and the mineralization of both relatively immature human bone marrow stromal preosteoblasts and mature human osteoblasts.
我们研究了骨形态发生蛋白-2（BMP-2）对人骨髓基质细胞（HBMSC）和人成骨细胞（HOB）中骨基质蛋白表达及其增殖和矿化的影响。HBMSC和HOB均表达BMP-2/-4的I型和II型受体。在β-甘油磷酸酯和抗坏血酸存在的情况下，用BMP-2处理这两种细胞类型4周会导致其基质矿化。BMP-2可提高两种细胞类型中碱性磷酸酶的mRNA水平和活性，并提高骨桥蛋白、骨唾液蛋白、骨钙素和α1（I）胶原的mRNA水平及蛋白质合成。虽然核心蛋白聚糖的mRNA水平升高，但双糖链蛋白聚糖的mRNA浓度未因BMP-2而改变。未观察到对骨连接蛋白有影响。BMP-2对骨基质蛋白表达的影响在25至100 ng/ml范围内呈剂量依赖性，且在处理1 - 7天后明显。在BMP-2存在的情况下，无论是在无血清条件下还是有血清存在时，HBMSC和HOB的增殖均降低。因此，BMP-2对相对未成熟的人骨髓基质前成骨细胞和成熟人成骨细胞的增殖、大多数骨基质蛋白的表达以及矿化都有深远影响。
