Sato R, Nakajima N, Soeta S, Sato J, Naito Y
Department of Veterinary Internal Medicine, Faculty of Agriculture, Iwate University, Morioka, Japan.
Am J Vet Res. 1997 Nov;58(11):1197-200.
To measure urine N-acetyl-beta-D-glucosaminidase (NAG) activity of healthy cattle, using 3 substrates (4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide, sodio-m-cresolsulfonphthaleinyl-N-acetyl-beta-D-glucosaminid e, and p-nitrophenyl-N-acetyl-beta-D-glucosaminide), and to determine the relations between the obtained values and age and sex of cattle.
50 healthy lactating Holstein-Friesian cows and 10 healthy Holstein-Friesian steers.
Untimed urine samples were collected, and urine NAG activity was measured, using the 3 aforementioned methods. Urine creatinine concentration also was measured, and NAG activity was expressed as units per gram of creatinine (NAG index). Correlations between urine NAG activity and age and sex of cattle were investigated. Furthermore, correlations among data obtained by each of the 3 methods were determined.
Urine NAG activity in cows measured by each of the 3 methods was < 3.0 U/L. Urine NAG activity in steers was significantly higher than that in cows. However, there was no significant difference between the sexes in NAG index. There were no significant differences in mean values of NAG activity and index among cows of various age groups. Individual values of urine NAG activity determined by each method correlated significantly with each other.
Urine NAG activity and NAG index of healthy cattle will be useful for determining diagnostic criteria of renal disease in cattle.
使用3种底物（4-甲基伞形酮基-N-乙酰-β-D-氨基葡萄糖苷、间甲酚磺酞钠基-N-乙酰-β-D-氨基葡萄糖苷和对硝基苯基-N-乙酰-β-D-氨基葡萄糖苷）测量健康牛的尿N-乙酰-β-D-氨基葡萄糖苷酶（NAG）活性，并确定所得值与牛的年龄和性别的关系。
50头健康的泌乳荷斯坦-弗里生奶牛和10头健康的荷斯坦-弗里生公牛。
收集非定时尿液样本，使用上述3种方法测量尿NAG活性。还测量了尿肌酐浓度，NAG活性以每克肌酐的单位数表示（NAG指数）。研究了尿NAG活性与牛的年龄和性别的相关性。此外，还确定了3种方法各自获得的数据之间的相关性。
用3种方法测得的奶牛尿NAG活性均<3.0 U/L。公牛的尿NAG活性显著高于奶牛。然而，NAG指数在性别之间没有显著差异。不同年龄组奶牛的NAG活性和指数平均值没有显著差异。每种方法测定的尿NAG活性个体值之间显著相关。
健康牛的尿NAG活性和NAG指数将有助于确定牛肾脏疾病的诊断标准。
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