Chae C
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Kyounggi Do, Republic of Korea.
J Vet Med Sci. 1997 Oct;59(10):931-4. doi: 10.1292/jvms.59.931.
Distinct glycoconjugate expression between dome enterocytes and villus enterocytes in ileum from twelve 3-week-old conventional pigs was examined by the use of twenty one biotinylated lectins with avidin-biotin-peroxidase complex method. Three patterns of staining were seen. First, lectins bind to dome enterocyte and villus enterocyte to approximately equal staining, i.e. DSL, WGA, s-WGA and ConA. Second, lectins display a markedly higher affinity for villus enterocyte than dome enterocyte, i.e. DBA, SBA, RCA I, SJA, VVA, BSL II, LEL, PNA, Jacalin, and ECL. Third, lectins exhibit negative staining to dome enterocyte only, i.e. PSA, LCA, UEA-I, and STL. Four lectins; PSA, LCA, UEA-I, STL may be useful negative marker to differentiate glycoconjugate expression between dome enterocyte and villus enterocyte. The staining patterns are slightly different among these negative markers. Three lectins, PSA, LCA, and UEA-1, are negative marker to differentiate dome enterocytes and villus enterocytes. But STL is also negative to dome epithelial surface and moderately positive to villus brush border. It is suggested that the present lectin-binding studies provide the marker of dome enterocyte as compared with villus enterocytes.
运用抗生物素蛋白-生物素-过氧化物酶复合物法，使用21种生物素化凝集素，检测了12头3周龄普通猪回肠中穹窿肠上皮细胞和绒毛肠上皮细胞之间不同的糖缀合物表达情况。观察到三种染色模式。第一，凝集素与穹窿肠上皮细胞和绒毛肠上皮细胞结合，染色程度大致相等，即DSL、WGA、s-WGA和ConA。第二，凝集素对绒毛肠上皮细胞的亲和力明显高于穹窿肠上皮细胞，即DBA、SBA、RCA I、SJA、VVA、BSL II、LEL、PNA、Jacalin和ECL。第三，凝集素仅对穹窿肠上皮细胞呈阴性染色，即PSA、LCA、UEA-I和STL。四种凝集素；PSA、LCA、UEA-I、STL可能是区分穹窿肠上皮细胞和绒毛肠上皮细胞糖缀合物表达的有用阴性标记物。这些阴性标记物的染色模式略有不同。三种凝集素，PSA、LCA和UEA-1，是区分穹窿肠上皮细胞和绒毛肠上皮细胞的阴性标记物。但STL对穹窿上皮表面也呈阴性，对绒毛刷状缘呈中度阳性。提示目前的凝集素结合研究提供了与绒毛肠上皮细胞相比的穹窿肠上皮细胞标记物。
