两个鸡POU家族基因的分离与定位以及与人类和小鼠的同线群鉴定。

Isolation and mapping of two chicken POU family genes and the identification of a syntenic group with human and mouse.

作者信息

Heltemes L M, Tuggle C K, Lamont S J

机构信息

Program of Immunobiology, Iowa State University, Ames 50011, USA.

出版信息

Anim Genet. 1997 Oct;28(5):346-50. doi: 10.1111/j.1365-2052.1997.00160.x.

DOI:10.1111/j.1365-2052.1997.00160.x

PMID:9363595

Abstract

Two partial chicken POU domain genes were isolated by genomic library screening and reverse transcriptase-polymerase chain reaction (RT-PCR). A cDNA clone encoding the POU domain of Brn-3a, which showed near identity to the human Brn-3a sequence (100% amino acid identity), was isolated and mapped to chicken linkage group E48. A Skn-1/Epoc-1/Oct-11 genomic clone was identified and mapped to chicken linkage group E49. This mapping identified a syntenic group in chicken linkage group E49 that was conserved with human chromosome 11q23 and mouse chromosome 9.

摘要

通过基因组文库筛选和逆转录聚合酶链反应（RT-PCR）分离出两个部分鸡POU结构域基因。分离出一个编码Brn-3a的POU结构域的cDNA克隆，其与人类Brn-3a序列显示出近乎相同的序列（100%氨基酸同一性），并将其定位到鸡连锁群E48。鉴定出一个Skn-1/Epoc-1/Oct-11基因组克隆，并将其定位到鸡连锁群E49。这种定位确定了鸡连锁群E49中的一个同线群，该同线群与人类11号染色体q23和小鼠9号染色体保守。