小胶质细胞和星形胶质细胞有一个共同的祖细胞。
Microglia and astroglia have a common progenitor cell.
作者信息
Fedoroff S, Zhai R, Novak J P
机构信息
Department of Anatomy and Cell Biology, University of Saskatchewan, Saskatoon, Canada.
出版信息
J Neurosci Res. 1997 Nov 1;50(3):477-86. doi: 10.1002/(SICI)1097-4547(19971101)50:3<477::AID-JNR14>3.0.CO;2-3.
Disaggregated neopallial cells from newborn C3H/HeJ mice were cloned in Grenier hybridoma tissue culture dishes, and culture wells that contained only one cell were marked. After 8-10 days of culturing, the cultures were fixed and double immunolabeled for microglia with Mac-1 antibody and for astroglia with antibody to GFAP. Each marked well containing a clone was identified as either a microglia, astroglia, mixed microglia-astroglia, or an unlabeled clone. The effect of LM cell line conditioned medium (LM-CM), which contains colony-stimulating factor-1, on the development of mixed microglia-astroglia clones was determined. Formation of mixed clones was dose dependent (P < 0.0001). We concluded that microglia and astroglia have a common progenitor cell and that the development of mixed clones is LM-CM dependent.
从新生C3H/HeJ小鼠中分离出的新皮质细胞在格氏杂交瘤组织培养皿中进行克隆，并标记仅含有一个细胞的培养孔。培养8 - 10天后，将培养物固定，并用抗Mac - 1抗体对小胶质细胞进行双重免疫标记，用抗GFAP抗体对星形胶质细胞进行双重免疫标记。每个含有克隆的标记孔被鉴定为小胶质细胞、星形胶质细胞、小胶质细胞 - 星形胶质细胞混合克隆或未标记克隆。确定了含有集落刺激因子 - 1的LM细胞系条件培养基（LM - CM）对小胶质细胞 - 星形胶质细胞混合克隆发育的影响。混合克隆的形成呈剂量依赖性（P < 0.0001）。我们得出结论，小胶质细胞和星形胶质细胞有一个共同的祖细胞，并且混合克隆的发育依赖于LM - CM。
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