Chouicha N, Lanotte G, Pratlong F, Cuba Cuba C A, Velez I D, Dedet J P
Laboratoire d'Ecologie Médicale et Pathologie Parasitaire, Faculté de Médecine, Montpellier, France.
Parasitology. 1997 Oct;115 ( Pt 4):343-8. doi: 10.1017/s0031182097001376.
Biochemical characterization of 137 Leishmania braziliensis isolates from South and Central America, and from selected endemic foci in Bolivia, Brazil and Colombia, performed by isoenzymatic electrophoresis using 10 enzymatic systems, showed a high enzymatic polymorphism (44 zymodemes obtained) based on the variation of a small number of enzymes. Cladistic analysis showed close links between the zymodemes within the L. braziliensis s.s. cluster. The position of 2 Colombian zymodemes obtained (MON204 and MON205) justify the inclusion of L. peruviana within the L. braziliensis cluster.
通过使用10种酶系统的同工酶电泳对来自南美洲和中美洲以及玻利维亚、巴西和哥伦比亚选定地方病灶的137株巴西利什曼原虫分离株进行生化特性分析，结果显示基于少数几种酶的变异存在高度酶多态性（获得44种酶型）。分支分析表明，巴西利什曼原虫狭义聚类中的酶型之间存在密切联系。所获得的2种哥伦比亚酶型（MON204和MON205）的位置证明将秘鲁利什曼原虫纳入巴西利什曼原虫聚类是合理的。
