Seong S Y, Kim H R, Huh M S, Park S G, Kang J S, Han T H, Choi M S, Chang W H, Kim I S
Department of Microbiology, Seoul National University College of Medicine, Chongno-gu, South Korea.
Vaccine. 1997 Nov;15(16):1741-7. doi: 10.1016/s0264-410x(97)00112-6.
Anti-oriential antibody inhibits Orientia tsutsugamushi attachment to, and penetration of, host cells. However, O. tsutsugamushi antigens that induce the production of a neutralizing antibody have not been identified. The authors immunized mice and rabbits with the recombinant 56 kDa protein of O. tsutsugamushi fused to the maltose binding protein of Escherichia coli (MBP-Bor56) and analysed their effect on O. tsutsugamushi attachment to or penetration of L929 cells. O. tsutsugamushi attachment and penetration were measured by using an indirect immunofluorescent antibody assay (IFA). O. tsutsugamushi growth in L929 cells was determined by [3H]thymidine uptake assay. By IFA, we observed a 96% reduction of attachment or penetration of O. tsutsugamushi treated with rabbit anti-MBP-Bor56 sera. [3H]thymidine uptake showed that mouse anti-MBP-Bor56 sera caused a 91% reduction in O. tsutsugamushi growth, when compared to mouse anti-MBP sera. These results suggest that the 56 kDa protein of O. tsutsugamushi plays an important role in O. tsutsugamushi attachment to or penetration of cells.
抗东方体抗体可抑制恙虫病东方体附着并穿透宿主细胞。然而，尚未鉴定出可诱导产生中和抗体的恙虫病东方体抗原。作者用与大肠杆菌麦芽糖结合蛋白（MBP-Bor56）融合的恙虫病东方体重组56 kDa蛋白免疫小鼠和兔子，并分析其对恙虫病东方体附着或穿透L929细胞的影响。采用间接免疫荧光抗体试验（IFA）检测恙虫病东方体的附着和穿透情况。通过[3H]胸腺嘧啶核苷摄取试验测定恙虫病东方体在L929细胞中的生长情况。通过IFA，我们观察到用兔抗MBP-Bor56血清处理的恙虫病东方体的附着或穿透减少了96%。[3H]胸腺嘧啶核苷摄取显示，与小鼠抗MBP血清相比，小鼠抗MBP-Bor56血清使恙虫病东方体的生长减少了91%。这些结果表明，恙虫病东方体的56 kDa蛋白在恙虫病东方体附着或穿透细胞过程中起重要作用。
