Delbrück S, Sonneborn A, Gerads M, Grablowitz A H, Ernst J F
Institut für Mikrobiologie, Heinrich-Heine Universität, Düsseldorf, Germany.
Yeast. 1997 Oct;13(13):1199-210. doi: 10.1002/(SICI)1097-0061(199710)13:13<1199::AID-YEA167>3.0.CO;2-J.
Genes encoding the Candida albicans ribosomal proteins L39 and S7 (RPL39, RPS7) were isolated and sequenced. From RPL39 cDNA a single intron interrupting the fifth codon in the genomic sequence could be deduced. Two homologous RPL39 genes in Saccharomyces cerevisiae contain a single intron in a conserved position. In contrast, C. albicans RPS7 was found to lack an intron, while both S. cerevisiae homologs are interrupted by single introns. The deduced L39 and S7 proteins contained 67% and 83% identical residues compared to the S. cerevisiae homologs. During hyphal induction the RPL39, RPS7 and RPL29 transcript levels increased three- to six-fold relative to ribosomal RNA, while ACT1 and RPS33 control transcripts were not regulated extensively. As suggested by unaltered transcript stabilities during hyphal induction, this regulation occurs on the transcriptional level; a conserved 18 bp palindromic sequence (5'-TTAGGGCTATAGCCCTAA-3'), which is present in the promoter regions of the RPL39 and RPS7 genes, may be involved in regulation.
编码白色念珠菌核糖体蛋白L39和S7（RPL39、RPS7）的基因被分离并测序。从RPL39 cDNA可以推断出在基因组序列中打断第五个密码子的单个内含子。酿酒酵母中的两个同源RPL39基因在保守位置含有单个内含子。相比之下，发现白色念珠菌RPS7缺乏内含子，而酿酒酵母的两个同源物都被单个内含子打断。与酿酒酵母的同源物相比，推导的L39和S7蛋白含有67%和83%的相同残基。在菌丝诱导过程中，RPL39、RPS7和RPL29转录水平相对于核糖体RNA增加了三到六倍，而ACT1和RPS33对照转录本没有受到广泛调节。正如菌丝诱导过程中转录本稳定性未改变所表明的，这种调节发生在转录水平；存在于RPL39和RPS7基因启动子区域的一个保守的18 bp回文序列（5'-TTAGGGCTATAGCCCTAA-3'）可能参与调节。
