[以肿瘤病毒为模型的细胞代谢与细胞外结构平行研究方法]
[Method of parallel study of cell metabolism and extracellular structures using oncornavirus as a model].
作者信息
Zaĭtseva O V, Nosik N N, Berezina O N, Ershov F I
出版信息
Vopr Virusol. 1976 Mar-Apr(2):169-73.
A method is described which permits simultaneous comparative study of cell metabolism and extracellular virus structures in minimal volumes. The method is based on the growing of cells in vials for scintillation count and it has been tested on a HEp-2 cell culture chronically producing oncornavirus type D. A comparative analysis of acid-insoluble radioactivity in heterogenous systems has been shown to be justified. For demonstration of the fields of possible utilization of the method, data are presented from the studies of the effect of various inhibitors (ethidium bromide, chloramphenicol, cycloheximide and anti-PPLO agent) on 3H-uridine incorporation into cells and extracellular virus structures.
本文描述了一种方法，该方法允许在最小体积下同时对细胞代谢和细胞外病毒结构进行比较研究。该方法基于在小瓶中培养细胞以进行闪烁计数，并已在长期产生D型肿瘤病毒的HEp-2细胞培养物上进行了测试。对异质系统中酸不溶性放射性的比较分析已被证明是合理的。为了证明该方法可能的应用领域，本文给出了各种抑制剂（溴化乙锭、氯霉素、环己酰亚胺和抗胸膜肺炎微生物剂）对3H-尿苷掺入细胞和细胞外病毒结构影响的研究数据。
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