Chan P K, Peiris J S, Yuen K Y, Liang R H, Lau Y L, Chen F E, Lo S K, Cheung C Y, Chan T K, Ng M H
Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Hong Kong.
J Med Virol. 1997 Nov;53(3):295-305. doi: 10.1002/(sici)1096-9071(199711)53:3<295::aid-jmv20>3.0.co;2-f.
Human cytomegalovirus (HCMV), human herpesvirus-6 (HHV-6), and human herpesvirus-7 (HHV-7) DNA in peripheral blood leukocytes (PBL) of 61 bone marrow transplant recipients was monitored weekly during the first 12 weeks post-transplantation by a nested polymerase chain reaction (PCR). Thirty-seven (61%), 17 (28%), and 32 (53%) of patients had one or more PBL specimens positive for HCMV, HHV-6 or HHV-7 DNA, respectively. HHV-7 DNA in PBL during the early post-transplant period was associated with a longer time to neutrophil engraftment (mean 28.8 days vs 19.8 days; P = 0.01). In two patients who failed to engraft, HHV-6 DNA and HHV-7 DNA was detected in plasma and PBL, respectively, early in their post-transplant period. Patients with HCMV disease were more likely to have concurrent HHV-7 DNA in PBL prior to onset of disease than were patients with asymptomatic HCMV infection, suggesting that HHV-7 may be a cofactor in the progression from HCMV infection to HCMV disease. In the 17 patients (179 specimens) in whom viral DNA in plasma was studied (in addition to PBL), a positive result was found only in 3. In each, viral DNA in plasma appeared to correlate with clinically significant disease. HHV-7 DNA in plasma was associated with encephalitis in an allograft recipient.
在61例骨髓移植受者移植后的前12周内，每周通过巢式聚合酶链反应（PCR）监测其外周血白细胞（PBL）中的人巨细胞病毒（HCMV）、人疱疹病毒6型（HHV-6）和人疱疹病毒7型（HHV-7）DNA。分别有37例（61%）、17例（28%）和32例（53%）患者的一份或多份PBL标本检测出HCMV、HHV-6或HHV-7 DNA呈阳性。移植后早期PBL中的HHV-7 DNA与中性粒细胞植入时间延长有关（平均28.8天对19.8天；P = 0.01）。在2例未植入的患者中，移植后早期分别在血浆和PBL中检测到HHV-6 DNA和HHV-7 DNA。与无症状HCMV感染患者相比，HCMV疾病患者在疾病发作前PBL中更易同时存在HHV-7 DNA，这表明HHV-7可能是HCMV感染进展为HCMV疾病的一个辅助因素。在17例（179份标本）同时研究血浆（除PBL外）病毒DNA的患者中，仅3例呈阳性结果。在每例中，血浆中的病毒DNA似乎与具有临床意义的疾病相关。血浆中的HHV-7 DNA与一名同种异体移植受者的脑炎有关。
