Dufossé L, Féron G, Latrasse A, Guichard E, Spinnler H E
IUP-IIA, Université de Bretagne Occidentale, Quimper, France.
Chirality. 1997;9(7):667-71. doi: 10.1002/(SICI)1520-636X(1997)9:7<667::AID-CHIR5>3.0.CO;2-4.
Sporidiobolus salmonicolor is an aroma-producing yeast which gives a peach-like smell to the culture media. The enantiomeric ratios of the five gamma-lactones produced by this yeast cultivated in two different media were determined by multidimensional gas chromatography (MDGC) on a fused silica capillary column coupled to a modified beta-cyclodextrin column. These ratios remain constant during growth and are not affected by the composition of the medium. The (R)-enantiomer is highly predominant (99%) for gamma-decalactone and predominant (68-88%) for gamma-octalactone, gamma-nonalactone, and (Z6)-gamma-dodecenolactone. A ratio close to racemic was found for gamma-dodecalactone. A discussion on the metabolic origin of these lactones is based on the analysis of the enantiomeric ratios obtained. With respect to consumers' preference for products considered as "natural," microbial lactone production may represent a valuable alternative to fruit flavors. The enantiomeric lactone ratios produced by Sporidiobolus salmonicolor are compared with those reported from some fruits.
鲑色掷孢酵母是一种能产生香气的酵母，可使培养基散发出类似桃子的气味。通过在与改性β-环糊精柱相连的熔融石英毛细管柱上进行多维气相色谱（MDGC），测定了在两种不同培养基中培养的这种酵母产生的五种γ-内酯的对映体比例。这些比例在生长过程中保持恒定，且不受培养基成分的影响。对于γ-癸内酯，（R）-对映体高度占主导（99%），对于γ-辛内酯、γ-壬内酯和（Z6）-γ-十二碳烯内酯，（R）-对映体占主导（68-88%）。发现γ-十二烷内酯的比例接近外消旋。基于所获得的对映体比例分析，对这些内酯的代谢来源进行了讨论。考虑到消费者对被视为“天然”产品的偏好，微生物内酯生产可能是水果风味的一种有价值的替代方案。将鲑色掷孢酵母产生的对映体内酯比例与一些水果报道的比例进行了比较。
