Yoshida M, Yokoo H, Nakahara K, Tomita M, Hamada N, Ishikawa M, Hatakeyama J, Tanaka M, Nagatsu I
Department of Pharmacology, Kurume University School of Medicine, Japan.
Brain Res. 1997 Sep 5;767(2):356-60. doi: 10.1016/s0006-8993(97)00474-5.
Infusion of muscimol (5 X 10[-5] M, 60 min) into the nucleus accumbens (NAC) through a dialysis membrane caused a significant increase in extracellular dopamine (DA) and its metabolite, 3,4-dihydroxyphenylacetic acid (DOPAC). Fos-like immunoreactivity induced by intra-NAC infusion of muscimol was seen ipsilaterally in many accumbofugal target areas, but no Fos-positive neurons were seen in the vicinity of the dialysis membrane in the NAC. Sequential staining of Fos and tyrosine hydroxylase (TH) immunoreactivities revealed that a portion of A10 dopaminergic neurons were double-labelled. These results suggest that muscimol in the NAC disinhibits mesolimbic DA neuronal activity possibly through activity of the accumbofugal GABA neuron system.
通过透析膜向伏隔核（NAC）注入蝇蕈醇（5×10[-5] M，60分钟）会导致细胞外多巴胺（DA）及其代谢产物3,4-二羟基苯乙酸（DOPAC）显著增加。向NAC内注入蝇蕈醇诱导的Fos样免疫反应在许多伏隔核传出靶区域同侧可见，但在NAC中透析膜附近未见Fos阳性神经元。Fos和酪氨酸羟化酶（TH）免疫反应的顺序染色显示，一部分A10多巴胺能神经元呈双标记。这些结果表明，NAC中的蝇蕈醇可能通过伏隔核传出GABA神经元系统的活动解除中脑边缘DA神经元活动的抑制。
