Dubnicoff T, Valentine S A, Chen G, Shi T, Lengyel J A, Paroush Z, Courey A J
Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, California 90095, USA.
Genes Dev. 1997 Nov 15;11(22):2952-7. doi: 10.1101/gad.11.22.2952.
The Dorsal morphogen acts as both an activator and a repressor of transcription in the Drosophila embryo to regulate the expression of dorsal/ventral patterning genes. Circumstantial evidence has suggested that Dorsal is an intrinsic activator and that additional factors (corepressors) convert it into a repressor. These corepressors, however, have previously eluded definitive identification. We show here, via the analysis of embryos lacking the maternally encoded Groucho corepressor and via protein-binding assays, that recruitment of Groucho to the template by protein:protein interactions is required for the conversion of Dorsal from an activator to a repressor. Groucho is therefore a critical component of the dorsal/ventral patterning system.
背侧形态发生素在果蝇胚胎中作为转录激活因子和抑制因子，调节背腹模式基因的表达。间接证据表明，背侧蛋白是一种内在激活因子，其他因子（共抑制因子）将其转化为抑制因子。然而，这些共抑制因子此前尚未得到明确鉴定。我们通过分析缺乏母源编码的Gro共抑制因子的胚胎以及蛋白质结合试验表明，通过蛋白质-蛋白质相互作用将Gro招募到模板上是背侧蛋白从激活因子转化为抑制因子所必需的。因此，Gro是背腹模式系统的关键组成部分。
