血小板衍生生长因子α受体启动子在转基因小鼠中的时空特异性
Temporal and spatial specificity of PDGF alpha receptor promoter in transgenic mice.
作者信息
Reinertsen K K, Bronson R T, Stiles C D, Wang C
机构信息
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA, USA.
出版信息
Gene Expr. 1997;6(5):301-14.
Aberrant expression of the platelet-derived growth factor alpha receptor (PDGF alpha R) has been linked to developmental abnormalities in vertebrate models, and has been implicated in multiple disease states in humans. To identify cis-acting regulatory elements that dictate expression of this receptor, we generated transgenic mice bearing the reporter gene beta-galactosidase (lacZ) under the control of a 6-kb promoter sequence. Expression of lacZ was monitored throughout embryonic development, with special focus on nervous tissue, skeleton, and several organ systems wherein PDGF alpha R expression is thought to play a pivotal role. In several independent transgenic mouse strains, lacZ expression recapitulated predominant features of PDGF alpha R gene expression during mouse development. These results demonstrate that critical tissue-specific regulatory elements for PDGF alpha R expression are located within a 6-kb upstream region of the PDGF alpha R gene.
血小板衍生生长因子α受体（PDGFαR）的异常表达与脊椎动物模型中的发育异常有关，并且在人类的多种疾病状态中也有涉及。为了鉴定决定该受体表达的顺式作用调控元件，我们构建了转基因小鼠，其携带在6 kb启动子序列控制下的报告基因β-半乳糖苷酶（lacZ）。在整个胚胎发育过程中监测lacZ的表达，特别关注神经组织、骨骼和几个器官系统，其中PDGFαR的表达被认为起着关键作用。在几个独立的转基因小鼠品系中，lacZ表达重现了小鼠发育过程中PDGFαR基因表达的主要特征。这些结果表明，PDGFαR表达的关键组织特异性调控元件位于PDGFαR基因上游6 kb的区域内。
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