Welte T, Koch F, Schuler G, Lechner J, Doppler W, Heufler C
Institut für Medizinische Chemie und Biochemie, University of Innsbruck, Austria.
Eur J Immunol. 1997 Oct;27(10):2737-40. doi: 10.1002/eji.1830271038.
Cytokine-mediated signaling pathways were studied in mouse dendritic cells (DC) by analysis of the activation pattern of STAT factors. Electrophoretic mobility shift assays were performed to detect STAT isoform-specific complexes. Granulocyte-macrophage colony-stimulating factor (GM-CSF) simultaneously induced complexes containing STAT1, STAT3, STAT5A, STAT5B and STAT6. In non-DC, a similar broad activation pattern of STAT factors by GM-CSF or other cytokines has not been observed so far. By comparison, in peritoneal macrophages, GM-CSF induced a complex with the properties of a truncated form of STAT5. Other cytokines tested on DC either failed to induce STAT factors [interleukin (IL)-1 beta, IL-2, IL-15], or activated the same STAT factors as observed in peritoneal macrophages (IL-4, IFN-gamma). Our results implicate a specific effect of GM-CSF on STAT signaling in DC which might account for the cell type-specific effect of this cytokine on development and function.
通过分析信号转导和转录激活因子（STAT）的激活模式，在小鼠树突状细胞（DC）中研究了细胞因子介导的信号通路。进行电泳迁移率变动分析以检测STAT异构体特异性复合物。粒细胞-巨噬细胞集落刺激因子（GM-CSF）同时诱导含有STAT1、STAT3、STAT5A、STAT5B和STAT6的复合物。迄今为止，在非DC中尚未观察到GM-CSF或其他细胞因子对STAT因子有类似的广泛激活模式。相比之下，在腹腔巨噬细胞中，GM-CSF诱导形成一种具有截短形式STAT5特性的复合物。在DC上测试的其他细胞因子要么未能诱导STAT因子[白细胞介素（IL）-1β、IL-2、IL-15]，要么激活与腹腔巨噬细胞中观察到的相同的STAT因子（IL-4、干扰素-γ）。我们的结果表明GM-CSF对DC中的STAT信号有特定作用，这可能解释了这种细胞因子对发育和功能的细胞类型特异性作用。
