Warchol M E
Department of Otolaryngology-HNS, School of Medicine, University of Virginia, Charlottesville 22908, USA.
J Neurobiol. 1997 Nov 20;33(6):724-34.
The factors that regulate the repair and regeneration of the sensory hair cells of the inner ear are not understood. Previous studies of hair cell injury in the lateral line sensory organs of amphibians and the cochleae of mammals have demonstrated that macrophages and other leukocytes are recruited to sites of hair cell lesions. The present study examined the distribution and activity of macrophages in organ cultures of the avian cochlea, a system whose regenerative abilities have been widely studied. Cochleae were removed from chicks and placed in organ culture, and precise hair cell lesions were created using a laser microbeam. Macrophages in the cultures were identified using histochemical, immunocytochemical, and morphologic criteria. It was found that (a) cultured cochleae contained a resident population of macrophages, and (b) increased numbers of macrophages were recruited to the sites of hair cell lesions. Furthermore, the latency of macrophage recruitment to lesions is consistent with a suggested role for macrophages in the initiation of hair cell regeneration.
调节内耳感觉毛细胞修复和再生的因素尚不清楚。先前对两栖动物侧线感觉器官和哺乳动物耳蜗毛细胞损伤的研究表明，巨噬细胞和其他白细胞会被募集到毛细胞损伤部位。本研究检测了鸟类耳蜗器官培养物中巨噬细胞的分布和活性，该系统的再生能力已得到广泛研究。从雏鸡中取出耳蜗并置于器官培养中，使用激光微束造成精确的毛细胞损伤。利用组织化学、免疫细胞化学和形态学标准鉴定培养物中的巨噬细胞。研究发现：（a）培养的耳蜗中存在常驻巨噬细胞群体；（b）更多的巨噬细胞被募集到毛细胞损伤部位。此外，巨噬细胞募集到损伤部位的延迟时间与巨噬细胞在毛细胞再生起始中所起的作用相符。
