Kalmykova A I, Dobritsa A A, Gvozdev V A
Department of Animal Molecular Genetics, Institute of Molecular Genetics, Russian Academy of Sciences, Moscow.
FEBS Lett. 1997 Oct 20;416(2):164-6. doi: 10.1016/s0014-5793(97)01195-2.
We report an exon-intron structure of the Su(Ste) repeat capable of encoding an isoform of the beta-subunit of protein kinase CK2. The predicted Su(Ste) gene product contains a drastically changed amino acid sequence of the N-terminal fragment as compared to the earlier described bCK2tes gene considered to be an ancestor of the Su(Ste) repeats. The following peculiarities of molecular divergence of the Su(Ste) and betaCK2tes genes are revealed: damages of the autophosphorylation site; usage of an alternative splicing site instead of a damaged one; conservation of the zinc finger domain in spite of local ORF alterations.
我们报道了一种Su(Ste)重复序列的外显子-内含子结构，它能够编码蛋白激酶CK2β亚基的一种异构体。与早期描述的被认为是Su(Ste)重复序列祖先的bCK2tes基因相比，预测的Su(Ste)基因产物的N端片段氨基酸序列发生了显著变化。揭示了Su(Ste)和βCK2tes基因分子差异的以下特点：自磷酸化位点的损伤；使用替代剪接位点替代受损位点；尽管局部开放阅读框发生改变，但锌指结构域仍保持保守。
