黑腹果蝇Y染色体上的Su(Ste)串联重复序列被转录并进行了不同的加工。
Su(Ste) diverged tandem repeats in a Y chromosome of Drosophila melanogaster are transcribed and variously processed.
作者信息
Kalmykova A I, Dobritsa A A, Gvozdev V A
机构信息
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow.
出版信息
Genetics. 1998 Jan;148(1):243-9. doi: 10.1093/genetics/148.1.243.
Abstract
We report the organization and transcription of diverged tandemly repeated Y-linked Su(Ste) genes that are considered as suppressors of testis-expressed X-linked-repeated Stellate genes that encode a protein sharing extensive homology with beta-subunit of casein kinase 2. Clustering of restriction variants is confirmed. Size variants of Su(Ste) repeats appeared to be nonhomogeneously distributed among the P1 phage clones. Different ways of Su(Ste) RNA processing because of the appearance of new splice sites and polyadenylation signals were detected. The high extent of homology between Stellate and Su(Ste) repeats suggested a possibility of Stellate suppression by antisense transcription of Su(Ste) elements. The detection of only "sense" Su(Ste) cDNAs in testis cDNA library allows us to reject this proposal. The genomic and cDNA clones are shown to be equally diverged. This indicates widespread rather than restricted transcription capacity of these repeats.
摘要
我们报道了Y染色体上串联重复的分化型Su(Ste)基因的组织与转录情况，这些基因被认为是睾丸表达的X染色体重复Stellate基因的抑制因子，后者编码一种与酪蛋白激酶2的β亚基具有广泛同源性的蛋白质。限制变体的聚类得到了证实。Su(Ste)重复序列的大小变体似乎在P1噬菌体克隆中呈非均匀分布。由于新剪接位点和聚腺苷酸化信号的出现，检测到了不同的Su(Ste) RNA加工方式。Stellate和Su(Ste)重复序列之间的高度同源性表明，Su(Ste)元件的反义转录有可能抑制Stellate。在睾丸cDNA文库中仅检测到“有义”的Su(Ste) cDNA，这使我们否定了这一推测。基因组克隆和cDNA克隆显示出同样程度的分化。这表明这些重复序列具有广泛而非受限的转录能力。
相似文献
1
Su(Ste) diverged tandem repeats in a Y chromosome of Drosophila melanogaster are transcribed and variously processed.黑腹果蝇Y染色体上的Su(Ste)串联重复序列被转录并进行了不同的加工。
Genetics. 1998 Jan;148(1):243-9. doi: 10.1093/genetics/148.1.243.
2
Molecular evolution of two paralogous tandemly repeated heterochromatic gene clusters linked to the X and Y chromosomes of Drosophila melanogaster.与黑腹果蝇X和Y染色体相关的两个串联重复的旁系同源异染色质基因簇的分子进化。
Mol Biol Evol. 2000 May;17(5):697-702. doi: 10.1093/oxfordjournals.molbev.a026348.
3
Structural organization and diversification of Y-linked sequences comprising Su(Ste) genes in Drosophila melanogaster.果蝇中包含抑制棒眼基因（Su(Ste)）的Y连锁序列的结构组织与多样化。
Nucleic Acids Res. 1992 Jul 25;20(14):3731-6. doi: 10.1093/nar/20.14.3731.
4
Acquisition and amplification of a testis-expressed autosomal gene, SSL, by the Drosophila Y chromosome.果蝇Y染色体对一个睾丸表达的常染色体基因SSL的获取与扩增。
Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6297-302. doi: 10.1073/pnas.94.12.6297.
5
Structure of the Y chromosomal Su(Ste) locus in Drosophila melanogaster and evidence for localized recombination among repeats.黑腹果蝇Y染色体上的Su(Ste)位点结构及重复序列间局部重组的证据。
Genetics. 1996 Jan;142(1):149-61. doi: 10.1093/genetics/142.1.149.
6
Paralogous stellate and Su(Ste) repeats: evolution and ability to silence a reporter gene.同源的星状重复序列和Su(Ste)重复序列：进化及沉默报告基因的能力。
Genetica. 2000;109(1-2):131-40. doi: 10.1023/a:1026596419250.
7
Double-stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in the D. melanogaster germline.双链RNA介导的黑腹果蝇生殖系中基因组串联重复序列和转座元件的沉默。
Curr Biol. 2001 Jul 10;11(13):1017-27. doi: 10.1016/s0960-9822(01)00299-8.
8
The Su(Ste) repeat in the Y chromosome and betaCK2tes gene encode predicted isoforms of regulatory beta-subunit of protein kinase CK2 in Drosophila melanogaster.果蝇Y染色体上的Su(Ste)重复序列和betaCK2tes基因编码预测的蛋白激酶CK2调节性β亚基的亚型。
FEBS Lett. 1997 Oct 20;416(2):164-6. doi: 10.1016/s0014-5793(97)01195-2.
9
10
An alien promoter capture as a primary step of the evolution of testes-expressed repeats in the Drosophila melanogaster genome.异源启动子捕获是黑腹果蝇基因组中睾丸表达重复序列进化的首要步骤。
Mol Biol Evol. 2005 Jul;22(7):1555-60. doi: 10.1093/molbev/msi147. Epub 2005 Apr 13.
引用本文的文献
1
Spatially revealed roles for lncRNAs in Drosophila spermatogenesis, Y chromosome function and evolution.长链非编码 RNA 在果蝇精子发生、Y 染色体功能和进化中的空间显现作用。
Nat Commun. 2024 May 7;15(1):3806. doi: 10.1038/s41467-024-47346-w.
2
A maternally programmed intergenerational mechanism enables male offspring to make piRNAs from Y-linked precursor RNAs in Drosophila.一种母系编程的跨代机制使雄性后代能够在果蝇中从 Y 连锁前体 RNA 产生 piRNAs。
Nat Cell Biol. 2023 Oct;25(10):1495-1505. doi: 10.1038/s41556-023-01227-4. Epub 2023 Sep 18.
3
Drosophila Protein Kinase CK2: Genetics, Regulatory Complexity and Emerging Roles during Development.果蝇蛋白激酶CK2：遗传学、调控复杂性及发育过程中的新作用
Pharmaceuticals (Basel). 2016 Dec 29;10(1):4. doi: 10.3390/ph10010004.
4
Endogenous non-retroviral RNA virus elements evidence a novel type of antiviral immunity.内源性非逆转录RNA病毒元件证明了一种新型抗病毒免疫。
Mob Genet Elements. 2016 Mar 22;6(3):e1165785. doi: 10.1080/2159256X.2016.1165785. eCollection 2016 May-Jun.
5
How do y-chromosomes modulate genome-wide epigenetic States: genome folding, chromatin sinks, and gene expression.Y染色体如何调节全基因组表观遗传状态：基因组折叠、染色质汇聚和基因表达。
J Genomics. 2014 May 1;2:94-103. doi: 10.7150/jgen.8043. eCollection 2014.
6
Population dynamics of PIWI-interacting RNAs (piRNAs) and their targets in Drosophila.果蝇中 PIWI 相互作用 RNA（piRNAs）及其靶标的种群动态。
Genome Res. 2010 Feb;20(2):212-27. doi: 10.1101/gr.095406.109. Epub 2009 Nov 30.
7
The biogenesis and function of PIWI proteins and piRNAs: progress and prospect.PIWI 蛋白和 piRNAs 的生物发生和功能：进展与展望。
Annu Rev Cell Dev Biol. 2009;25:355-76. doi: 10.1146/annurev.cellbio.24.110707.175327.
8
Evidence for rolling circle replication of tandem genes in Drosophila.果蝇中串联基因滚环复制的证据。
Nucleic Acids Res. 2005 Aug 9;33(14):4519-26. doi: 10.1093/nar/gki764. Print 2005.
9
Extrachromosomal circular DNA of tandemly repeated genomic sequences in Drosophila.果蝇中串联重复基因组序列的染色体外环状DNA。
Genome Res. 2003 Jun;13(6A):1133-45. doi: 10.1101/gr.907603.
本文引用的文献
1
Further evidence consistent with Stellate's involvement in meiotic drive.与星状细胞参与减数分裂驱动相一致的进一步证据。
Genetics. 1996 Feb;142(2):641-3. doi: 10.1093/genetics/142.2.641.
2
Structure of the Y chromosomal Su(Ste) locus in Drosophila melanogaster and evidence for localized recombination among repeats.黑腹果蝇Y染色体上的Su(Ste)位点结构及重复序列间局部重组的证据。
Genetics. 1996 Jan;142(1):149-61. doi: 10.1093/genetics/142.1.149.
3
Drosophila melanogaster male germ line-specific transcripts with autosomal and Y-linked genes.具有常染色体和Y连锁基因的黑腹果蝇雄性生殖系特异性转录本。
Genetics. 1993 May;134(1):293-308. doi: 10.1093/genetics/134.1.293.
4
Human casein kinase II. The subunit alpha protein activates transcription of the subunit beta gene.人酪蛋白激酶II。α亚基蛋白激活β亚基基因的转录。
J Biol Chem. 1993 Mar 15;268(8):5694-702.
5
Inducibility and negative autoregulation of CREM: an alternative promoter directs the expression of ICER, an early response repressor.CREM的诱导性和负向自动调节：一个替代启动子指导早期反应抑制因子ICER的表达。
Cell. 1993 Dec 3;75(5):875-86. doi: 10.1016/0092-8674(93)90532-u.
6
A fertility region on the Y chromosome of Drosophila melanogaster encodes a dynein microtubule motor.黑腹果蝇Y染色体上的一个育性区域编码一种动力蛋白微管马达。
Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11132-6. doi: 10.1073/pnas.90.23.11132.
7
Genetic analysis of Stellate elements of Drosophila melanogaster.黑腹果蝇星状元件的遗传分析。
Genetics. 1994 Dec;138(4):1181-97. doi: 10.1093/genetics/138.4.1181.
8
Protein kinases. 4. Protein kinase CK2: an enzyme with multiple substrates and a puzzling regulation.蛋白激酶。4. 蛋白激酶CK2：一种具有多种底物且调控方式令人费解的酶。
FASEB J. 1995 Mar;9(5):313-23. doi: 10.1096/fasebj.9.5.7896000.
9
The Ste locus, a component of the parasitic cry-Ste system of Drosophila melanogaster, encodes a protein that forms crystals in primary spermatocytes and mimics properties of the beta subunit of casein kinase 2.Ste基因座是黑腹果蝇寄生性cry-Ste系统的一个组成部分，它编码一种在初级精母细胞中形成晶体并模拟酪蛋白激酶2β亚基特性的蛋白质。
Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):6067-71. doi: 10.1073/pnas.92.13.6067.
10
Cytogenetic analysis of a segment of the Y chromosome of Drosophila melanogaster.黑腹果蝇Y染色体一段的细胞遗传学分析。
Genetics. 1984 Aug;107(4):591-610. doi: 10.1093/genetics/107.4.591.
Zotero 插件