Carneiro C M, Krasilnikov O V, Yuldasheva L N, Campos de Carvalho A C, Nogueira R A
Center of Biological Sciences, Department of Biophysics and Radiobiology, Federal University of Pernambuco, Recife, PE, Brazil.
FEBS Lett. 1997 Oct 20;416(2):187-9. doi: 10.1016/s0014-5793(97)01198-8.
The mammalian porin channel (VDAC, porin-31BM) was reconstituted in planar lipid bilayers under voltage clamp conditions. The radii of both entrances of the channel were examined using a method that consisted in filling the channel with different non-electrolytes through its cis or trans entrances while recording single channel conductances. As a result it was found that the geometry of channels formed by porin-31BM could not be approximated by a perfectly cylindrical pore. In fact there is an asymmetry in the geometry of the channel: the diameters of the cis and trans entrances were estimated to be approximately 2 nm and approximately 4 nm respectively.
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