van Gemeren I A, Punt P J, Drint-Kuyvenhoven A, Broekhuijsen M P, van't Hoog A, Beijersbergen A, Verrips C T, van den Hondel C A
Department of Molecular and Cellular Biology, University of Utrecht, The Netherlands.
Gene. 1997 Oct 1;198(1-2):43-52. doi: 10.1016/s0378-1119(97)00290-4.
We describe the cloning and characterisation of the BiP gene homologues of the filamentous fungi Aspergillus niger and Aspergillus awamori. The BiP genes of these black Aspergilli encode an identical protein of 672 amino acids, which has a high homology with the BiP protein from Saccharomyces cerevisiae and contains a putative signal sequence of 38 amino acids. The DNA sequences of the Aspergillus BiP genes diverge in particular in the three intronic sequences and the 5'- and 3'- noncoding regions. Sequences resembling Heat Shock Elements (HSE) and Unfolded Protein Response (UPR) elements, as found in the yeast KAR2 promoter, are present in the 5' non-transcribed regions of both genes. The expression of the A. niger bipA gene is increased by heat shock and tunicamycin treatment.
我们描述了丝状真菌黑曲霉和泡盛曲霉BiP基因同源物的克隆与特性。这些黑曲霉的BiP基因编码一种由672个氨基酸组成的相同蛋白质，该蛋白质与酿酒酵母的BiP蛋白具有高度同源性，并包含一个由38个氨基酸组成的推定信号序列。曲霉BiP基因的DNA序列在三个内含子序列以及5'和3'非编码区尤其不同。在两个基因的5'非转录区都存在类似于酵母KAR2启动子中发现的热休克元件（HSE）和未折叠蛋白反应（UPR）元件的序列。黑曲霉bipA基因的表达通过热休克和衣霉素处理而增加。
