Yesodi V, Hauschner H, Tabib Y, Firon N
Department of Plant Genetics, Institute of Field and Garden Crops The Volcani Center, A. R. O., P. O. B. 6 Bet Dagan 50250, Israel.
Curr Genet. 1997 Nov;32(5):348-57. doi: 10.1007/s002940050287.
The gene copies for the alpha-subunit of the mitochondrial F1ATPase (atpA) were isolated and characterized in both male-fertile and cytoplasmic male sterile (CMS) petunia. Two copies, an intact gene and a truncated gene, were detected in both cytoplasms. The accumulated data, based upon a comparison of the sequences (the open reading frames as well as the 5' and 3' flanking regions) of the two atpA copies, both in male-fertile and CMS Petunia, indicate that: (1) they differ in their genomic organization and (2) a common progenitor cytoplasm, containing two copies of an intact atpA sequence, served as the origin for the atpA copies of the fertility and CMS-inducing cytoplasms. Homologous recombination through the progenitor intact atpA sequences is assumed to have caused the rearrangement in the 3' portion of the atpA open reading frame and the generation of the truncated atpA gene. It is thus suggested that the atpA pseudogenes, in both male-fertile and CMS cytoplasms, originated from a common progenitor atpA pseudogene sequence.
在线粒体F1ATP酶（atpA）α亚基的基因拷贝在雄性可育和细胞质雄性不育（CMS）矮牵牛中被分离和鉴定。在两种细胞质中均检测到两个拷贝，一个完整基因和一个截短基因。基于对雄性可育和CMS矮牵牛中两个atpA拷贝的序列（开放阅读框以及5'和3'侧翼区域）比较所积累的数据表明：（1）它们在基因组组织上存在差异；（2）一个包含完整atpA序列两个拷贝的共同祖先细胞质，是育性和CMS诱导细胞质中atpA拷贝的起源。推测通过祖先完整的atpA序列进行同源重组导致了atpA开放阅读框3'部分的重排以及截短atpA基因的产生。因此，有人提出，在雄性可育和CMS细胞质中，atpA假基因均起源于一个共同的祖先atpA假基因序列。
