Schneider G B
Am J Anat. 1976 May;146(1):93-100. doi: 10.1002/aja.1001460105.
The diameter of lymphocytes, isolated from a sample of peripheral blood by Ficoll-Hypaque sedimentation, was determined after eight different steps in the preparative procedure for scanning electron microscopy (SEM). There was a 43% total reduction in mean cellular diameter of the lymphocytes studied, the diameter dropping from 8.4 mum prior to fixation, to a final diameter of 4.8 mum in the cells examined under the scanning electron microscope. The bulk of this shrinkage occurred during critical-point drying. In view of this finding, caution should be exercised in describing detailed cell surface morphology by SEM when similar preparative procedures are employed.
通过Ficoll-泛影葡胺沉降法从外周血样本中分离出的淋巴细胞，在扫描电子显微镜（SEM）制备程序的八个不同步骤后测定其直径。所研究的淋巴细胞平均细胞直径总共减少了43%，直径从固定前的8.4微米降至在扫描电子显微镜下检查的细胞的最终直径4.8微米。这种收缩大部分发生在临界点干燥过程中。鉴于这一发现，当采用类似的制备程序时，在通过SEM描述详细的细胞表面形态时应谨慎。
