Katayama I, Schneider G B
Am J Pathol. 1977 Jan;86(1):163-82.
Studies with transmission and scanning electron microscopes (TEM and SEM) were performed on specimens from patients suspected of leukemic reticuloendotheliosis (LRE), irrespective of the final diagnoses. The blood samples were fixed immediately in order to avoid in vitro changes of leukocytes. Leukemic reticuloendotheliosis cells demonstrated numerous long microvilli and pseudopods, whereas the immediately fixed lymphocytes demonstrated fewer, shorter microvilli and no pseudopods. These surface features distinguishing LRE cells from lymphocytes and monocytes were appreciated by both SEM and TEM. In addition, TEM revealed significant differences in the incidence of certain ultrastructural alterations. Whereas nuclear pockets were rare in lre cells, they were relatively frequent in lymphocytes of other hematologic disorders. The inclusions of ribosome-lamella complexes were not only more prevalent in patients with LRE but were seen in a higher percentage of leukemic cells from patients with LRE than in those from patients with other hematologic disorders. Electron microscopy can play a role in the laboratory diagnosis of LRE.
使用透射电子显微镜和扫描电子显微镜（TEM和SEM）对疑似白血病性网状内皮增生症（LRE）患者的标本进行了研究，无论最终诊断结果如何。血液样本立即固定，以避免白细胞在体外发生变化。白血病性网状内皮增生症细胞显示出许多长微绒毛和伪足，而立即固定的淋巴细胞显示出较少、较短的微绒毛且没有伪足。扫描电子显微镜和透射电子显微镜都能识别出这些将LRE细胞与淋巴细胞和单核细胞区分开来的表面特征。此外，透射电子显微镜揭示了某些超微结构改变发生率的显著差异。核袋在LRE细胞中很少见，而在其他血液系统疾病的淋巴细胞中相对常见。核糖体-板层复合体的包涵体不仅在LRE患者中更普遍，而且在LRE患者的白血病细胞中出现的比例高于其他血液系统疾病患者的白血病细胞。电子显微镜可在LRE的实验室诊断中发挥作用。
