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鉴定在陆生蜗牛中枢神经系统D组神经元中特异性表达的两个新基因。

Identification of two novel genes specifically expressed in the D-group neurons of the terrestrial snail CNS.

作者信息

Balaban P M, Zakharov I S, Poteryaev D A, Belyavsky A V

机构信息

Engelhardt Institute of Molecular Biology, Moscow, Russia.

出版信息

Invert Neurosci. 1996 Jun;2(1):61-9. doi: 10.1007/BF02336661.

DOI:10.1007/BF02336661
PMID:9372156
Abstract

A search for genes specifically expressed in the giant interneurons of parietal ganglia of the snail Helix lucorum yielded, among others, two genes named HDS1 and HDS2. According to data obtained by Northern hybridization and whole-mount in situ hybridization, both genes are neurospecific and expressed almost exclusively in the peptidergic D-group neurons (Sakharov, 1974) located in the right parietal ganglion. In situ hybridization of the HDS1 and HDS2 probes with CNS of several related species of the Helicoidea superfamily identified in all cases similarly located homologous groups of neurons. Sequencing of the near full-length cDNA copies of the HDS1 and HDS2 genes revealed open reading frames 107 and 102 amino acids long for HDS1 and HDS2, respectively. Both putative proteins contain a hydrophobic leader peptide and putative recognition sites for furin-like and PC-like endopeptidases. Predicted amino acid sequences of the HDS1 and HDS2 proteins were found to be moderately homologous to each other, as well as to the LYCP preprohormone expressed by the light yellow cells of the freshwater snail Lymnaea stagnalis. These results confirm an earlier hypothesis that the D-group of the Helix family and the light yellow cells of Lymnaea stagnalis represent homologous neuronal groups. Our data suggest that the HDS1 and HDS2 genes encode precursors of secreted molecules, most likely neuropeptides or neurohormones.

摘要

对光亮蜗牛顶神经节中的巨大中间神经元中特异性表达的基因进行搜索,除其他基因外,得到了两个名为HDS1和HDS2的基因。根据通过Northern杂交和全组织原位杂交获得的数据,这两个基因都是神经特异性的,几乎只在位于右顶神经节的肽能D组神经元中表达(萨哈罗夫,1974年)。用HDS1和HDS2探针与螺旋超科几个相关物种的中枢神经系统进行原位杂交,在所有情况下都鉴定出了位置相似的同源神经元群。对HDS1和HDS2基因的近全长cDNA拷贝进行测序,结果显示HDS1和HDS2的开放阅读框分别为107和102个氨基酸长。两种推定的蛋白质都含有一个疏水前导肽以及推定的弗林样和PC样内肽酶识别位点。发现HDS1和HDS2蛋白质的预测氨基酸序列彼此之间以及与淡水蜗牛椎实螺的浅黄色细胞表达的LYCP前激素适度同源。这些结果证实了一个早期的假设,即螺旋科的D组和椎实螺的浅黄色细胞代表同源神经元群。我们的数据表明,HDS1和HDS2基因编码分泌分子的前体,很可能是神经肽或神经激素。

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本文引用的文献

1
Protein targeting in the neuron.神经元中的蛋白质靶向
Annu Rev Neurosci. 1993;16:95-127. doi: 10.1146/annurev.ne.16.030193.000523.
2
Spatial segregation of odorant receptor expression in the mammalian olfactory epithelium.哺乳动物嗅觉上皮中气味受体表达的空间隔离。
Cell. 1993 Jul 30;74(2):309-18. doi: 10.1016/0092-8674(93)90422-m.
3
The isolation of a cDNA encoding a neuropeptide prohormone from the light yellow cells of Lymnaea stagnalis.从椎实螺的浅黄色细胞中分离出一种编码神经肽前激素的cDNA。
Cell Mol Neurobiol. 1993 Jun;13(3):263-70. doi: 10.1007/BF00733754.
4
Storage and release of neurotransmitters.神经递质的储存与释放。
Cell. 1993 Jan;72 Suppl:43-53. doi: 10.1016/s0092-8674(05)80027-3.
5
Novel gene HCS1 is specifically expressed in the giant interneurones of the terrestrial snail.新型基因HCS1在陆生蜗牛的巨大中间神经元中特异性表达。
Neuroreport. 1994 Jan 31;5(5):589-92. doi: 10.1097/00001756-199401000-00014.
6
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
Anal Biochem. 1983 Jul 1;132(1):6-13. doi: 10.1016/0003-2697(83)90418-9.
7
Physiological and pharmacological identification of neurons in the central nervous system of Helix pomatia L.苹果螺(Helix pomatia L.)中枢神经系统中神经元的生理和药理学鉴定
Acta Physiol Acad Sci Hung. 1969;35(1):19-30.
8
Ultrastructure and histochemistry of neurosecretory cells and neurohaemal areas in the pond snail Lymnaea stagnalis (L.).静水椎实螺(Lymnaea stagnalis (L.))神经分泌细胞和神经血器官的超微结构与组织化学
Z Zellforsch Mikrosk Anat. 1970;108(2):190-224. doi: 10.1007/BF00335295.
9
A new method for predicting signal sequence cleavage sites.一种预测信号序列切割位点的新方法。
Nucleic Acids Res. 1986 Jun 11;14(11):4683-90. doi: 10.1093/nar/14.11.4683.
10
Proteolytic maturation of insulin is a post-Golgi event which occurs in acidifying clathrin-coated secretory vesicles.胰岛素的蛋白水解成熟是一种发生在高尔基体后、在酸化的网格蛋白包被分泌小泡中进行的事件。
Cell. 1987 Jun 19;49(6):865-8. doi: 10.1016/0092-8674(87)90624-6.