Krist L F, Koenen H, Calame W, van der Harten J J, van der Linden J C, Eestermans I L, Meyer S, Beelen R H
Department of Anesthesiology, AMC, Amsterdam, The Netherlands.
Anat Rec. 1997 Nov;249(3):399-404. doi: 10.1002/(SICI)1097-0185(199711)249:3<399::AID-AR11>3.0.CO;2-J.
Milky spots in the human greater omentum are preformed specific accumulations of primarily macrophages within the stroma of the greater omentum. To obtain a better understanding of milky spots in the human greater omentum, the development and the earliest forms of milky spots in the human greater omentum were studied, with special attention to the macrophage population.
Specimens of human greater omentum were obtained from fetuses of 20 to 40 weeks gestation and one newborn three days old (n = 6). Using mature macrophages (RFD 7), activated macrophages (RFD 1), B-lymphocytes (CD 22), and T-lymphocytes (CD 2), and immunoperoxydase labeling, the percentage of these cells in developing milky spots and the development of milky spots were studied by light microscopy. A time-dependent increase in the percentage of positive staining cells and the size of clusters was analyzed using the non-parametric Spearman rank correlation test.
Small accumulations of cells with about 50% monocytes/macrophages were present at 20 weeks of gestation. With increasing gestational age the number of clusters of cells increased significantly (P < 0.01) as well as their size (P < 0.01). Starting at 29 weeks, vascularized clusters of cells were seen; true milky spots were present at 35 weeks. A significant (P < 0.05) increase in the percentage of mature macrophages was found in developing milky spots, whereas no activated macrophages were seen. The percentage of B-lymphocytes and T-lymphocytes found in the clusters of cells and milky spots increased significantly (P < 0.05) but did not exceed 10% of the total number of cells.
From our data it can be concluded that milky spots are specific structures in the greater omentum formed between the 20th and 35th week of gestation. Further, we concluded that immature cells (promonocytes) mature locally in developing milky spots.
人大网膜中的乳斑是大网膜基质内主要由巨噬细胞预先形成的特定聚集物。为了更好地了解人大网膜中的乳斑，对人 大网膜中乳斑的发育及最早形式进行了研究，特别关注巨噬细胞群体。
从妊娠 20 至 40 周的胎儿及 1 例出生 3 天的新生儿获取人 大网膜标本（n = 6）。使用成熟巨噬细胞（RFD 7）、活化巨噬细胞（RFD 1）、B 淋巴细胞（CD 22）和 T 淋巴细胞（CD 2），采用免疫过氧化物酶标记法，通过光学显微镜研究这些细胞在发育中的乳斑中的百分比及乳斑的发育情况。使用非参数 Spearman 秩相关检验分析阳性染色细胞百分比和细胞簇大小随时间的增加情况。
妊娠 20 周时存在少量细胞聚集物，其中约 50%为单核细胞/巨噬细胞。随着孕周增加，细胞簇数量显著增加（P < 0.01），其大小也显著增加（P < 0.01）。从 29 周开始可见血管化的细胞簇；35 周时出现真正的乳斑。在发育中的乳斑中，成熟巨噬细胞百分比显著增加（P < 0.05），而未见活化巨噬细胞。在细胞簇和乳斑中发现的 B 淋巴细胞和 T 淋巴细胞百分比显著增加（P < 0.05），但不超过细胞总数的 10%。
根据我们的数据可以得出结论，乳斑是妊娠第 20 至 35 周之间在大网膜中形成的特定结构。此外，我们得出结论，未成熟细胞（前单核细胞）在发育中的乳斑中局部成熟。
