Krist L F, Kerremans M, Broekhuis-Fluitsma D M, Eestermans I L, Meyer S, Beelen R H
Department of Surgery, Academic Hospital Vrije Universiteit, Amsterdam, The Netherlands.
Cancer Immunol Immunother. 1998 Dec;47(4):205-12. doi: 10.1007/s002620050522.
The role that milky spots in the greater omentum play in tumour cell spread in the peritoneal cavity is presently not fully understood. To study whether intraperitoneally injected tumour cells appear preferentially in milky spots of the greater omentum and to study the changes in the greater omentum, and especially in the cell population of milky spots after tumour cell infiltration, the following study was performed. A detailed temporal sequences of changes in morphology and cellular composition in milky spots of the greater omentum of Wag/Rij rats 5, 15, 30, 60 min, 2, 4, 8, 16, 24 h, 2, 4, 8 days and 2 and 4 weeks after intraperitoneal administration of 2.0 x 10(6) CC 531 tumour cells was investigated by light microscopy and electron microscopy (pre-embedding labelling). Our data showed that the milky spots in the greater omentum were the sites to which tumour cells migrated preferentially from the peritoneal cavity. The tumour cells infiltrated the milky spots and formed clusters within. The cellular population in milky spots reacted by a very rapid influx of young macrophages during the first hour and an increase of the total number of cells (P < 0.01). After 4 h tumour cells were also located on the greater omentum outside the area of the milky spots. Around these tumour cell deposits, new milky spots are formed, which increased the total number of milky spots. The cells present in milky spots are not capable of reversing the growth of tumours and finally a solid omental cake of tumour cells is formed.
大网膜乳斑在肿瘤细胞在腹腔内扩散中所起的作用目前尚未完全明确。为研究腹腔注射的肿瘤细胞是否优先出现在大网膜乳斑中，以及研究大网膜尤其是肿瘤细胞浸润后乳斑细胞群的变化，我们开展了以下研究。通过光学显微镜和电子显微镜（包埋前标记），研究了Wag/Rij大鼠腹腔注射2.0×10(6)个CC 531肿瘤细胞后5分钟、15分钟、30分钟、60分钟、2小时、4小时、8小时、16小时、24小时、2天、4天、8天以及2周和4周时，大网膜乳斑形态和细胞组成变化的详细时间序列。我们的数据表明，大网膜乳斑是肿瘤细胞从腹腔优先迁移至的部位。肿瘤细胞浸润乳斑并在其中形成细胞簇。乳斑中的细胞群在最初1小时内通过年轻巨噬细胞的快速大量涌入以及细胞总数增加做出反应（P<0.01）。4小时后，肿瘤细胞也出现在乳斑区域外的大网膜上。在这些肿瘤细胞沉积周围，形成了新的乳斑，这增加了乳斑的总数。乳斑中的细胞无法逆转肿瘤的生长，最终形成了由肿瘤细胞构成的坚实网膜块。
